Expression of transcription factor Yin Yang 1 in human osteosarcomas

The transcription factor Yin Yang 1 (YY1) is known to be present in some human cancer cell lines and its expression correlates with immune-mediated apoptosis. By using Western blot analysis, we have shown that the YY1 protein is strongly expressed in human osteosarcoma cells and localised mainly in...

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Published inEuropean journal of cancer (1990) Vol. 42; no. 15; pp. 2420 - 2424
Main Authors de Nigris, Filomena, Botti, Chiara, de Chiara, Anna, Rossiello, Raffaele, Apice, Gaetano, Fazioli, Flavio, Fiorito, Carmela, Sica, Vincenzo, Napoli, Claudio
Format Journal Article
LanguageEnglish
Published Oxford Elsevier Ltd 01.10.2006
Elsevier
Subjects
Adolescent
Adult
Biological and medical sciences
Blotting, Western
Bone Neoplasms - metabolism
Bone Neoplasms - pathology
Cell Line, Tumor
Fluorescent Antibody Technique
Humans
Medical sciences
Neoplasm Proteins - metabolism
Osteosarcoma
Osteosarcoma - metabolism
Osteosarcoma - pathology
Pharmacology. Drug treatments
Reverse Transcriptase Polymerase Chain Reaction
Transcription factor
Tumors
YY1
YY1 Transcription Factor - metabolism
YY1
Transcription factor
Osteosarcoma
Osteoarticular system
Human
Signal transduction
Cancerology
Sarcoma
Diseases of the osteoarticular system
Pharmacology
Malignant tumor
Bone
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Summary:The transcription factor Yin Yang 1 (YY1) is known to be present in some human cancer cell lines and its expression correlates with immune-mediated apoptosis. By using Western blot analysis, we have shown that the YY1 protein is strongly expressed in human osteosarcoma cells and localised mainly in the nucleus. Moreover, by using immunohistochemistry and RT-PCR techniques, we have analysed the expression of YY1 protein in biopsies from human osteosarcomas. The YY1 protein was not detectable by immunohistochemistry in osteoid tissue. However, its expression was restricted to osteosarcoma tissues. These data were confirmed by densitometric analysis of RT-PCR for YY1 expression. Thus, YY1 gene activation appears to be an early event in the process of osteoblastic transformation and its detection may represent, together with the analysis of other established markers, a useful diagnostic tool in human osteosarcomas.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0959-8049
1879-0852
DOI:10.1016/j.ejca.2006.06.008