RASPBERRY3 Gene Encodes a Novel Protein Important for Embryo Development

• Published in: Plant physiology (Bethesda) Vol. 129; no. 2; pp. 691 - 705
• Main Authors: Apuya, Nestor R., Yadegari, Ramin, Fischer, Robert L., John H. Harada, Goldberg, Robert B.
• Format: Journal Article
• Language: English
• Published: United States American Society of Plant Biologists 01.06.2002; American Society of Plant Physiologists
• Subjects: Algae; Amino Acid Sequence; Arabidopsis - genetics; Arabidopsis - growth & development; Arabidopsis Proteins - genetics; Base Sequence; Chloroplasts; Chloroplasts - genetics; Cinnamates; Cloning, Molecular; Complementary DNA; Culture Techniques; Development and Hormone Action; DNA, Bacterial - genetics; DNA, Complementary - chemistry; DNA, Complementary - genetics; DNA, Complementary - isolation & purification; DNA, Plant - chemistry; DNA, Plant - genetics; Embryogenesis; Embryos; Fruits; Genes; Genetic Complementation Test; Genomics; Hygromycin B - analogs & derivatives; Hygromycin B - pharmacology; Kanamycin - pharmacology; Molecular Sequence Data; Mutation; Plant cells; Plant Leaves - genetics; Plants, Genetically Modified; Plasmids - genetics; Proteins; Seedlings; Seeds - genetics; Seeds - growth & development; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Transgenes
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.004010

We identified a new gene that is interrupted by T-DNA in an Arabidopsis embryo mutant called raspberry3. raspberry3 has "raspberry-like" cellular protuberances with an enlarged suspensor characteristic of other raspberry embryo mutants, and is arrested morphologically at the globular stage of embryo development. The predicted RASPBERRY3 protein has domains found in proteins present in prokaryotes and algae chloroplasts. Computer prediction analysis suggests that the RASPBERRY3 protein may be localized in the chloroplast. Complementation analysis supports the possibility that the RASPBERRY3 protein may be involved in chloroplast development. Our experiments demonstrate the important role of the chloroplast, directly or indirectly, in embryo morphogenesis and development.