Partial protection against infectious bursal disease virus through DNA-mediated vaccination with the VP2 capsid protein and chicken IL-2 genes

• Published in: Vaccine Vol. 22; no. 9; pp. 1249 - 1259
• Main Authors: Hulse, Diane J, Romero, Carlos H
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 12.03.2004; Elsevier; Elsevier Limited
• Subjects: Animals; Applied microbiology; Biological and medical sciences; Birnaviridae Infections - immunology; Birnaviridae Infections - pathology; Body Weight - physiology; Bursa of Fabricius - pathology; Capsid Proteins - genetics; Capsid Proteins - immunology; Chick Embryo; Chicken interleukin 2; Chickens - genetics; Chickens - immunology; DNA Vaccination; DNA, Viral - biosynthesis; DNA, Viral - genetics; Fluorescent Antibody Technique; Fundamental and applied biological sciences. Psychology; Genes; Hemorrhage - pathology; IBDV; Infectious bursal disease virus; Infectious bursal disease virus - immunology; Interleukin-2 - genetics; Interleukin-2 - immunology; Microbiology; Miscellaneous; Mortality; Neutralization Tests; Plasmids; Plasmids - immunology; Poultry; Spleen - pathology; T-Lymphocytes - immunology; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Vaccines, DNA - immunology; Virology; DNA Vaccination; IBDV; Chicken interleukin 2; Cytokine; Birnaviridae; Genetic vaccine; Protein; Virus; Vertebrata; Gene; Interleukin 2; Infectious bursal disease virus; Chicken; Aves; Veterinary; Capsid; Avibirnavirus
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2003.09.021

Several DNA vaccination experiments were performed to determine the protective capability of a plasmid DNA molecule encoding the VP2 capsid protein gene of infectious bursal disease virus (IBDV) injected into chickens in the presence or absence of chicken interleukin 2 (IL-2) plasmid DNA. The results of these experiments indicate that partial protection against IBDV can be achieved by using the VP2 gene of IBDV as a DNA vaccine. Furthermore, the simultaneous injection of chicken IL-2 plasmid DNA significantly increased the protection after challenge with the virulent strain. It was also found that immunological tolerance may have been induced in one of the chicken experiments by vaccination with plasmid DNA.