Neurochemistry of myenteric plexus neurons of bank vole (Myodes glareolus) ileum

• Published in: Research in veterinary science Vol. 95; no. 3; pp. 846 - 853
• Main Authors: Sadeghinezhad, J., Sorteni, C., Di Guardo, G., D’Agostino, C., Agrimi, U., Nonno, R., Chiocchetti, R.
• Format: Journal Article
• Language: English
• Published: England Elsevier India Pvt Ltd 01.12.2013; Elsevier Limited
• Subjects: Amino acids; Animals; Arvicolinae - metabolism; Arvicolinae - physiology; Bank vole; Calbindins - metabolism; Calcitonin Gene-Related Peptide - metabolism; Choline O-Acetyltransferase - metabolism; Enteric nervous system; Female; Fluorescent Antibody Technique, Indirect - veterinary; Immunohistochemistry; Laboratory animals; Male; Microscopy, Fluorescence - veterinary; Myenteric Plexus - metabolism; Myenteric Plexus - physiology; Myodes; Neurochemical code; Neurochemistry; Neurons; Nitric Oxide Synthase Type I - metabolism; Peptides; Prions; Rodents; Sheep; Studies; Substance P - metabolism; Veterinary medicine; Immunohistochemistry; Enteric nervous system; Bank vole; Neurochemical code
• ISSN: 0034-5288; 1532-2661
• DOI: 10.1016/j.rvsc.2013.07.028

The neurochemistry of enteric neurons differs among species of small laboratory rodents (guinea-pig, mouse, rat). In this study we characterized the phenotype of ileal myenteric plexus (MP) neuronal cells and fibers of the bank vole (Myodes glareolus), a common rodent living in Europe and in Northern Asia which is also employed in prion experimental transmission studies. Six neuronal markers were tested: choline acetyltransferase (ChAT), neuronal nitric oxide synthase (nNOS), calbindin (CALB), calcitonin gene-related peptide (CGRP) and substance P (SP), along with HuC/D as a pan-neuronal marker. Neurons expressing ChAT- and nNOS-immunoreactivity (IR) were 36±12% and 24±5%, respectively. Those expressing CGRP-, SP- and CALB-IR were 3±3%, 21±5% and 6±2%, respectively. Therefore, bank vole MPs differ consistently from murine MPs in neurons expressing CGRP-, SP- and CALB-IR. These data may contribute to define the prion susceptibility of neuron cell populations residing within ileal MPs from bank voles, along with their morpho-functional alterations following oral experimental prion challenge.