Circulating microRNA profiling identifies microRNAs linked to prediabetes associated with alcohol dependence syndrome

• Published in: Alcohol (Fayetteville, N.Y.) Vol. 122; pp. 101 - 109
• Main Authors: Ramaswamy, Palaniswamy, S V, Athira, Misra, Pratibha, Chauhan, V.S., Adhvaryu, Arka, Gupta, Anurodh, G, Ankita, M K, Sibin
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.02.2025; Elsevier Limited
• Subjects: Adult; Alcohol; Alcohol dependence syndrome; Alcohol use; Alcoholism - blood; Alcoholism - complications; Alcoholism - genetics; Biochemistry; Biomarkers; Biomarkers - blood; Case-Control Studies; Cholesterol; Circulating MicroRNA - blood; Circulating MicroRNA - genetics; Diabetes; Diabetes mellitus; DNA microarrays; Drug dependence; Family medical history; Female; Gene expression; Gene Expression Profiling - methods; Glucose; Growth factors; Health care; hsa-miR-3135b; hsa-miR-320b; Humans; Hybridization; Insulin resistance; Liver cirrhosis; Liver diseases; Male; Metabolic disorders; microarray; MicroRNAs; MicroRNAs - blood; Middle Aged; miRNA; Pancreatitis; Pituitary gland; prediabetes; Prediabetic State - blood; Prediabetic State - diagnosis; Prediabetic State - genetics; Psychiatric/Mental Health; Thyroid gland; United States > US; India; T2DM; Alcohol dependence syndrome; miRNAs; FBG; ADS Prediabetes; ADS; SD; miRNA; KEGG; HbA1c; RT-qPCR; prediabetes; CI; ROC; GO; IQR; hsa-miR-320b; PCA; microarray; hsa-miR-3135b; ADS NGT; NGT; HC; OGTT; ADA; oral glucose tolerance test; Reverse transcription-quantitative polymerase chain reaction; Type 2 diabetes mellitus; Standard deviation; Gene Ontology; fasting blood glucose; Normal glucose tolerance; ADS patients with normal glucose tolerance; Kyoto Encyclopedia of Genes and Genomes; Healthy Control; ADS patients with prediabetes; Confidence interval; Receiver operating characteristic; MicroRNAs; Interquartile range; American Diabetes Association; glycated haemoglobin; Principal component analysis
• ISSN: 0741-8329; 1873-6823; 1873-6823
• DOI: 10.1016/j.alcohol.2024.01.003

MicroRNAs are abundant in serum and have emerged as important regulators of gene expression, implicating them in a wide range of diseases. The purpose of this study was to discover and validate serum miRNAs in prediabetes associated with alcohol dependence syndrome (ADS). Serum samples from ADS patients with or without prediabetes and normoglycemic controls were subjected to microarray. Validation of identified candidate miRNAs was performed by RT-qPCR. Additionally, GO and KEGG pathway analyses were carried out to uncover target genes anticipated to be controlled by the candidate miRNAs. Notably, 198, and 172 miRNAs were differentially expressed in ADS-patients with or without prediabetes compared to healthy controls, and 7 miRNAs in ADS-patients with prediabetes compared to ADS-normoglycemic patients, respectively. Furthermore, hsa-miR-320b and hsa-miR-3135b were differentially expressed exclusively in ADS-patients with prediabetes, and this was further validated. Interestingly, GO and KEGG pathway analysis revealed that genes predicted to be modulated by the candidates were considerably enriched in numerous diabetes-related biological processes and pathways. Our findings revealed that ADS-patients with or without prediabetes have different sets of miRNAs compared to normoglycemic healthy subjects. We propose serum hsa-miR-320b and hsa-miR-3135b as potential biomarkers for the diagnosis of prediabetes in ADS-patients. •A study was conducted to discover and validate serum miRNAs in prediabetes associated with alcohol dependence syndrome (ADS), using microarray profiling and RT qPCR validation.•Seven miRNAs were differentially expressed in serum samples of ADS patients with prediabetes compared to normoglycemic ADS patients.•Hsa-miR-320b and hsa-miR-3135b were differentially expressed exclusively in ADS patients with prediabetes and this was consistent in the validation study.•GO and KEGG pathway studies revealed that genes predicted to be modulated by candidate miRNAs were involved in numerous related biological processes and pathways.•The study propose serum hsa-miR-320b and hsa-miR-3135b as potential biomarkers for the diagnosis of prediabetes associated with ADS.