Immunisation against PCV2 structural protein by DNA vaccination of mice

• Published in: Vaccine Vol. 22; no. 11; pp. 1358 - 1361
• Main Authors: Kamstrup, Søren, Barfoed, Annette Malene, Frimann, Tine Holland, Ladekjær-Mikkelsen, Anne-Sofie, Bøtner, Anette
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 29.03.2004; Elsevier; Elsevier Limited
• Subjects: Animals; Antibodies, Viral - analysis; Antibodies, Viral - biosynthesis; Applied microbiology; Biological and medical sciences; Circoviridae Infections - immunology; Circoviridae Infections - prevention & control; Circovirus - genetics; Circovirus - immunology; Cloning; Deoxyribonucleic acid; DNA; DNA, Viral - immunology; Female; Fundamental and applied biological sciences. Psychology; Genes; Genes, Viral - genetics; Hogs; Immunization; Immunoprophylaxis; Infections; Methods; Mice; Mice, Inbred BALB C; Microbiology; Miscellaneous; Open Reading Frames - genetics; Porcine circovirus 2; Porcine circovirus type 2; Postweaning multisystemic wasting syndrome; Proteins; Reverse Transcriptase Polymerase Chain Reaction; Swine; Swine Diseases - immunology; Swine Diseases - virology; Technological change; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Vaccines, DNA - genetics; Vaccines, DNA - immunology; Viral Structural Proteins - genetics; Viral Vaccines - genetics; Viral Vaccines - immunology; Virology; Postweaning multisystemic wasting syndrome; Immunoprophylaxis; Porcine circovirus type 2; Immunization; Rodentia; Cachexia; Porcine circovirus; Genetic vaccine; Protein; Virus; Vertebrata; Mammalia; Mouse; Circovirus; Circoviridae; Veterinary
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2004.01.032

Porcine circovirus type 2 (PCV2) is the causative agent of an emerging swine disease, postweaning multisystemic wasting syndrome (PMWS). The disease affects primarily 5–12-weeks-old pigs which might suggest that infection with PCV2 occurs when the level of maternal antibodies have declined to sub-protective levels around weaning at 3–5-weeks of age. If immunoprophylaxis is to be effective, an immunisation method capable of breaking through maternal immunity must be employed. In this study, we have developed and investigated the potential of a DNA vaccination approach to be one such method. The gene encoding the capsid protein of PCV2 was cloned in a DNA vaccination plasmid and expression of capsid protein was demonstrated in vitro. Mice were gene gun vaccinated three timesand all mice responded serologically by raising antibodies against PCV2. The results suggest, that DNA based vaccination might offer opportunities for vaccination of piglets against PCV2.