Molecular mechanism of 9-cis-retinoic acid inhibition of adipogenesis in 3T3-L1 cells

• Published in: Biochemical and biophysical research communications Vol. 433; no. 1; pp. 102 - 107
• Main Authors: Sagara, Chiaki, Takahashi, Katsuhiko, Kagechika, Hiroyuki, Takahashi, Noriko
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 29.03.2013
• Subjects: 3T3-L1 Cells; 60 APPLIED LIFE SCIENCES; 9-cis-Retinoic acid; Adipogenesis; Adipogenesis - drug effects; Adipogenesis - physiology; ALBUMINS; Animals; CATTLE; DEXAMETHASONE; Dibenzazepines - pharmacology; EDTA; ELECTROPHORESIS; GELS; INHIBITION; INSULIN; Lipid Metabolism - drug effects; LIPIDS; MICE; PH VALUE; PHOSPHATES; PPAR gamma - antagonists & inhibitors; PPAR gamma - metabolism; PPARγ; RECEPTORS; Receptors, Retinoic Acid - antagonists & inhibitors; Receptors, Retinoic Acid - metabolism; RETINOIC ACID; Retinoid X Receptor alpha - antagonists & inhibitors; Retinoid X Receptor alpha - metabolism; RXR; SODIUM CHLORIDES; SULFATES; TRANSCRIPTION FACTORS; Tretinoin - pharmacology; VITAMIN D; PBS; RAR; SDS; DTT; VDR; 9-cis-Retinoic acid; DIM; PAGE; EDTA; RXR; PPARγ; RA; BSA; PPAR; Adipogenesis
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2013.02.057

► We examined the effects of 9-cis-RA on adipogenesis in mouse preadipocyte 3T3-L1. ► 9-cis-RA inhibited lipid accumulation in adipogenetically-induced 3T3-L1 cells. ► A RXR pan-antagonist suppressed the inhibitory effects of 9-cis-RA on adipogenesis. ► This antagonist had no effects on RXRα and PPARγ levels in 9-cis-RA-treated cells. ► 9-cis-RA-induced decrease in both RXRα and PPARγ was independent of RXR activation. Retinoic acid (RA) signaling is mediated by specific nuclear hormone receptors. Here we examined the effects of 9-cis-RA on adipogenesis in mouse preadipocyte 3T3-L1 cells. 9-cis-RA inhibits the lipid accumulation of adipogenetically induced 3T3-L1 cells. The complex of retinoid X receptor α (RXRα) with peroxisome proliferator-activated receptor γ (PPARγ) is a major transcription factor in the process of adipogenesis, and the levels of these molecules were decreased by 9-cis-RA treatment. A RXR pan-antagonist suppressed 9-cis-RA’s inhibitory effects on adipogenesis, but not on the intracellular levels of both RXRα and PPARγ. These results suggest that 9-cis-RA could inhibit adipogenesis by activating RXR, and decrease both RXR and PPARγs levels in a RXR activation-independent manner.