Genetic Divergence between North American Ancestral Soybean Lines and Introductions with Resistance to Soybean Cyst Nematode Revealed by Chloroplast Haplotype

• Published in: The Journal of heredity Vol. 96; no. 5; pp. 593 - 599
• Main Authors: Bilyeu, K. D, Beuselinck, P. R
• Format: Journal Article
• Language: English
• Published: United States Oxford University Press 01.09.2005; Oxford Publishing Limited (England)
• Subjects: Animals; Breeding - methods; chloroplast DNA; cyst nematodes; DNA, Chloroplast - genetics; Genetic diversity; Genetic Variation; Genotype & phenotype; germplasm; germplasm conservation; germplasm evaluation; Glycine max; Glycine max - genetics; Glycine soja; haplotypes; Haplotypes - genetics; Heterodera glycines; Immunity, Innate - genetics; lines; Nematoda; Nematodes; North America; pest resistance; Plant Diseases - genetics; Plant Diseases - parasitology; plant genetic resources; Polymorphism; Polymorphism, Single Nucleotide - genetics; Soybeans; North America
• ISSN: 0022-1503; 1465-7333; 1471-8505
• DOI: 10.1093/jhered/esi087

Domesticated soybean [Glycine max (L.) Merr.] is a major crop with an established ancestral relationship to wild soybean (Glycine soja Sieb. & Zucc.) native to Asia. Soybean genetic diversity can be assessed at different levels by identification of polymorphic alleles at genetic loci, in either the plastid or nuclear genomes. The objective of this study was to evaluate genetic diversity based on chloroplast haplotypes for soybean genotypes present in the USDA germplasm resource collection. Shared chloroplast haplotypes represent broad groups of genetic relatedness. Previous work categorized three-quarters of the cultivated soybeans from Asia into a single haplotype group. Our results confirmed the close relationship of North American soybean ancestors and G. max plant introductions previously identified as representing potential sources of soybean genetic variation with the finding that these genotypes belonged to a single chloroplast haplotype group. Genetic diversity was identified in soybean genotypes determined to have a high density of single nucleotide polymorphisms and in a screen of accessions with resistance to soybean cyst nematode. Characterization of soybean plant introduction lines into chloroplast haplotype group may be an important initial step in evaluating the appropriate use of particular soybean genotypes.