The effect of magnetic field exposure on differentiation of magnetite nanoparticle-loaded adipose-derived stem cells

• Published in: Materials Science & Engineering C Vol. 109; p. 110652
• Main Authors: Labusca, Luminita, Herea, Dumitru-Daniel, Danceanu, Camelia-Mihaela, Minuti, Anca Emanuela, Stavila, Cristina, Grigoras, Marian, Gherca, Daniel, Stoian, George, Ababei, Gabriel, Chiriac, Horia, Lupu, Nicoleta
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2020; Elsevier BV
• Subjects: Adipogenesis; Adipose Tissue - cytology; Adipose Tissue - metabolism; Biocompatibility; Biomedical materials; Biotechnology; Cell Differentiation; Cell therapy; Differentiation (biology); Exposure; Humans; In vivo methods and tests; Magnetic Fields; Magnetic Iron Oxide Nanoparticles - chemistry; Magnetic nanoparticles; Magnetite; Magnetomechanical stimulation; Materials science; Mechanical stimuli; Mesenchyme; Nanoparticles; Nanotechnology; Osteoblasts; Osteogenesis; Osteoporosis; Regenerative medicine; Remote control; Stem cells; Stem Cells - cytology; Stem Cells - metabolism; Stimulation; Tissue engineering; Variable magnetic field; Variable magnetic field; Magnetomechanical stimulation; Cell therapy; Osteogenesis; Magnetic nanoparticles; Adipogenesis
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2020.110652

Magnetic nanoparticles (MNPs) are versatile tools for various applications in biotechnology and nanomedicine. MNPs-mediated cell tracking, targeting and imaging are increasingly studied for regenerative medicine applications in cell therapy and tissue engineering. Mechanical stimulation influences mesenchymal stem cell differentiation. Here we show that MNPs-mediated magneto-mechanical stimulation of human primary adipose derived stem cells (ADSCs) exposed to variable magnetic field (MF) influences their adipogenic and osteogenic differentiation. ADSCs loaded with biocompatible magnetite nanoparticles of 6.6 nm, and with an average load of 21 picograms iron/cell were exposed to variable low intensity (0.5 mT - LMF) and higher intensity magnetic fields (14.7 and 21.6 mT - HMF). Type, duration, intensity and frequency of MF differently affect differentiation. Short time (2 days) intermittent exposure to LMF increases adipogenesis while longer (7 days) intermittent as well as continuous exposure favors osteogenesis. HMF (21.6 mT) short time intermittent exposure favors osteogenesis. Different exposure protocols can be used to increase differentiation dependently on expected results. Magnetic remotely-actuated MNPs up-taken by ADSCs promotes the shift towards osteoblastic lineage. ADSCs-MNPs under MF exposure could be used for enabling osteoblastic conversion during cell therapy for systemic osteoporosis. Current results enable further in vivo studies investigating the role of remotely-controlled magnetically actuated ADSCs-MNPs for the treatment of osteoporosis. •Primary human adipose derived mesenchymal cells (ADSCs) loaded with magnetic nanoparticles (MNPs).•Setup for intermittent and continuous exposure of cells to low and high intensity magnetic fields (LMF and HMF).•Increased viability of ADSCs incubated with MNPs in MF compared to non-loaded counterparts.•MF parameters and the exposure way interfere with ADSCs differentiation in terms of adipogenic and osteogenic conversion.•Short time intermittent LMF favor adipogenesis while longer intermittent, continuous LMF as well as HMF favor osteogenesis.