Antimicrobial Effect of Chitosan Films on Food Spoilage Bacteria

• Published in: International journal of molecular sciences Vol. 22; no. 11; p. 5839
• Main Authors: Wrońska, Natalia, Katir, Nadia, Miłowska, Katarzyna, Hammi, Nisrine, Nowak, Marta, Kędzierska, Marta, Anouar, Aicha, Zawadzka, Katarzyna, Bryszewska, Maria, El Kadib, Abdelkrim, Lisowska, Katarzyna
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI 29.05.2021; MDPI AG
• Subjects: Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Anti-Infective Agents - chemistry; Anti-Infective Agents - pharmacology; antimicrobial activity; Bacterial Outer Membrane - drug effects; Biocompatible Materials; biodegradable material; Cell Membrane Permeability - drug effects; Chitosan - chemistry; chitosan modified films; cytotoxicity; Food Microbiology; Food Packaging; graphene fillers; Membranes, Artificial; metal-oxide clusters; Metals - chemistry; Polymers; biodegradable material; graphene fillers; antimicrobial activity; metal-oxide clusters; chitosan modified films; cytotoxicity
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms22115839

Synthetic materials commonly used in the packaging industry generate a considerable amount of waste each year. Chitosan is a promising feedstock for the production of functional biomaterials. From a biological point of view, chitosan is very attractive for food packaging. The purposes of this study were to evaluate the antibacterial activity of a set of chitosan-metal oxide films and different chitosan-modified graphene (oxide) films against two foodborne pathogens: ATCC 33560 and 19115. Moreover, we wanted to check whether the incorporation of antimicrobial constituents such as TiO , ZnO, Fe O , Ag, and graphene oxide (GO) into the polymer matrices can improve the antibacterial properties of these nanocomposite films. Finally, this research helps elucidate the interactions of these materials with eukaryotic cells. All chitosan-metal oxide films and chitosan-modified graphene (oxide) films displayed improved antibacterial ( ATCC 33560 and 19115) properties compared to native chitosan films. The CS-ZnO films had excellent antibacterial activity towards (90% growth inhibition). Moreover, graphene-based chitosan films caused high inhibition of both tested strains. Chitosan films with graphene (GO, GOP, GOP-HMDS, rGO, GO-HMDS, rGOP), titanium dioxide (CS-TiO 20:1a, CS-TiO 20:1b, CS-TiO 2:1, CS-TiO 1:1a, CS-TiO 1:1b) and zinc oxide (CS-ZnO 20:1a, CS-ZnO 20:1b) may be considered as a safe, non-cytotoxic packaging materials in the future.