Purification and characterization of cell growth factor in bovine colostrum

• Published in: Journal of Veterinary Medical Science Vol. 58; no. 1; pp. 47 - 53
• Main Authors: Kishikawa, Y. (Nihon Univ., Tokyo (Japan). Coll. of Agriculture and Veterinary Medicine), Watanabe, T, Kubo, S
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.01.1996; Japan Science and Technology Agency
• Subjects: 3T3 Cells; Amino Acids - analysis; Animals; Animals, Newborn; Biological Assay; bovine colostrum; calf kidney epithelial cell; CALOSTRO; Cattle; CELL CULTURE; Cell Division - drug effects; Cell Line; characterization; Chromatography, Gel; Chromatography, Ion Exchange; COLOSTRUM; Colostrum - chemistry; COWS; CRECIMIENTO; CROISSANCE; CULTIVO DE CELULAS; CULTURE DE CELLULE; DNA - biosynthesis; Dogs; EPITELIO; epithelial growth factor; EPITHELIUM; Female; GROWTH; Growth Substances - chemistry; Growth Substances - isolation & purification; Growth Substances - pharmacology; Humans; Insulin-Like Growth Factor I - chemistry; Insulin-Like Growth Factor I - pharmacology; Kidney; KIDNEYS; Mice; Milk, Human - chemistry; PURIFICACION; PURIFICATION; Rats; REIN; RINONES; Time Factors; VACA; VACHE
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.58.47

Bovine colostrum has growth factor activity for stimulating DNA synthesis in calf kidney epithelial cells (CKT-1), Madin-canine kidney epithelial cells (MDCK) and rat L6 myoblasts (L6), of which the DNA stimulation level and the activity change with the time elapsed after the birth of a calf varied with their respective cells. The growth factor activity of colostrum for CKT-1 was stable regardless of the collection time of colostrum, and it was purified about 3, 650-fold in an overall yield of 1.2% from colostrum obtained 30 min after the birth of a calf. The purified growth factor had a molecular weight (MW) of 5, 000 and an isoelectric point of pH 9.7, and the amino acid composition was: Asx5, Thr2, Ser4, Glx14, Pro2, Gly4, Ala4, Val2, Ile, Leu2, Tyr, Phe, Lys, His and Arg. The stimulated DNA synthesis in CKT-1 and L6 by the addition of purified growth factor at a final concentration of 16 ng/ml was as the same extent as calf serum at a final concentration of 1.52 mg/ml, and the relative activity for CKT-1 was even greater than that for L6.