Characterization, extraction and purification of lutein produced by an indigenous microalga Scenedesmus obliquus CNW-N
► Microalga Scenedesmus obliquus CNW-N is a suitable candidate for lutein production. ► The developed method shows higher selectivity for extracting lutein from microalgae. ► Optimizing saponfication conditions reduced the operation time from 3 to 6h. ► Low temperature and antioxidant addition led t...
Saved in:
Published in | Biochemical engineering journal Vol. 78; pp. 24 - 31 |
---|---|
Main Authors | , , , , , |
Format | Journal Article Conference Proceeding |
Language | English |
Published |
Amsterdam
Elsevier B.V
01.09.2013
Elsevier |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | ► Microalga Scenedesmus obliquus CNW-N is a suitable candidate for lutein production. ► The developed method shows higher selectivity for extracting lutein from microalgae. ► Optimizing saponfication conditions reduced the operation time from 3 to 6h. ► Low temperature and antioxidant addition led to higher storage stability for lutein.
This study aimed to improve the commercial viability of microalgae-based lutein production using an isolated microalga Scenedesmus obliquus CNW-N possessing a high lutein content of over 0.25%. Effective lutein extraction protocols, appropriate storage methods, and purification procedures were developed. Disruption of microalgae cells was most efficient with a bead-beater. The conventional saponification step was modified to reduce the overall extraction time by 24h. Diethyl ether exhibited the best lutein extraction efficiency. Storage of the lutein extract at low temperature (4 or −20°C) with antioxidant addition (around 0.01% BHT) can maintain 90% lutein stability after 80 days. Addition of a suitable amount of the antioxidant could promote the stability of lutein extracts under the exposure of light. The protocol developed in this work allows efficient lutein extraction from S. obliquus CNW-N at a lower cost. Further purification was employed to elevate the purity of lutein and its commercial value. |
---|---|
Bibliography: | http://dx.doi.org/10.1016/j.bej.2012.11.017 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1369-703X 1873-295X |
DOI: | 10.1016/j.bej.2012.11.017 |