Differences in cell kinetic changes among renal cancer cell lines treated with interferon-α

• Published in: International journal of urology Vol. 8; no. 8; pp. 449 - 454
• Main Authors: Kasuya, Yutaka, Hosaka, Yoshio, Matsushima, Hisashi, Goto, Toshitaka, Kitamura, Tadaichi
• Format: Journal Article
• Language: English
• Published: Melbourne, Australia Blackwell Science Pty 01.08.2001
• Subjects: Carcinoma, Renal Cell - pathology; Cell Cycle - drug effects; cyclin; DNA ploidy; DNA, Neoplasm - analysis; flow cytometry; Humans; Interferon-alpha - pharmacology; interferon-α; Kidney Neoplasms - pathology; Kinetics; renal cancer; Tumor Cells, Cultured
• ISSN: 0919-8172; 1442-2042
• DOI: 10.1046/j.1442-2042.2001.00346.x

Background: Interferon (IFN)‐α shows certain clinical effects on the treatment of renal cell carcinoma. The purpose of the present study was to investigate its direct effects and to compare the responses among different human renal cancer cell lines. Methods: Three cell lines, ACHN, RCC10RGB and OS‐RC‐2, were incubated with IFN‐α and evaluated using MTT assay for cell proliferation and two‐color flow cytometry for cell‐cycle‐specific cyclin expressions coupled with DNA ploidy analysis. Results: Interferon‐α inhibited cell proliferation and caused cell accumulation at S and G2/M phases. However, IFN‐α induced no significant change in cyclins D1, E, A or B1 expression. Interestingly, cell kinetic changes caused by IFN‐α were different among cell lines. Cell proliferation was suppressed most in ACHN, then RCC10RGB and least in OS‐RC‐2. Comparing DNA histograms, ACHN showed distinct increase of G2/M cells associated with elevation of late S cells. RCC10RGB showed a predominant increase of whole S cells accompanied with a slight increase of G2/M. OS‐RC‐2 showed a modest increase of S cells with a little change of G2/M cells. Chronological observation revealed that S‐phase increase and proliferative inhibition appeared on day 1 and day 3, respectively, in ACHN and RCC10RGB, and on day 5 in OS‐RC‐2. Conclusions: Interferon‐α induced substantial cell kinetic interference directly in the tested human renal carcinoma cell lines. The degree of change was different according to the nature of the cell line. It may partly indicate the variety of the efficacy of IFN‐α treatment against renal cancers.