The type IV secretion protein TraK from the Enterococcus conjugative plasmid pIP501 exhibits a novel fold

• Published in: Acta crystallographica. Section D, Biological crystallography. Vol. 70; no. 4; pp. 1124 - 1135
• Main Authors: Goessweiner-Mohr, Nikolaus, Fercher, Christian, Arends, Karsten, Birner-Gruenberger, Ruth, Laverde-Gomez, Diana, Huebner, Johannes, Grohmann, Elisabeth, Keller, Walter
• Format: Journal Article
• Language: English
• Published: 5 Abbey Square, Chester, Cheshire CH1 2HU, England International Union of Crystallography 01.04.2014; Wiley Subscription Services, Inc
• Subjects: antibiotic resistance; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; conjugative plasmid; DNA-Binding Proteins - chemistry; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Enterococcus; Enterococcus faecalis - chemistry; Enterococcus faecalis - genetics; Enterococcus faecalis - metabolism; Models, Molecular; pIP501; Plasmids - chemistry; Plasmids - metabolism; Protein Structure, Tertiary; type IV secretion; Enterococcus; conjugative plasmid; type IV secretion; antibiotic resistance; pIP501
• ISSN: 1399-0047; 0907-4449; 1399-0047
• DOI: 10.1107/S1399004714001606

Conjugative plasmid transfer presents a serious threat to human health as the most important means of spreading antibiotic resistance and virulence genes among bacteria. The required direct cell–cell contact is established by a multi‐protein complex, the conjugative type IV secretion system (T4SS). The conjugative core complex spans the cellular envelope and serves as a channel for macromolecular secretion. T4SSs of Gram‐negative (G−) origin have been studied in great detail. In contrast, T4SSs of Gram‐positive (G+) bacteria have only received little attention thus far, despite the medical relevance of numerous G+ pathogens (e.g. enterococci, staphylococci and streptococci). This study provides structural information on the type IV secretion (T4S) protein TraK of the G+ broad host range Enterococcus conjugative plasmid pIP501. The crystal structure of the N‐terminally truncated construct TraKΔ was determined to 3.0 Å resolution and exhibits a novel fold. Immunolocalization demonstrated that the protein localizes to the cell wall facing towards the cell exterior, but does not exhibit surface accessibility. Circular dichroism, dynamic light scattering and size‐exclusion chromatography confirmed the protein to be a monomer. With the exception of proteins from closely related T4SSs, no significant sequence or structural relatives were found. This observation marks the protein as a very exclusive, specialized member of the pIP501 T4SS.