Treatment of Surgically Induced Acute Liver Failure with Transplantation of Highly Differentiated Immortalized Human Hepatocytes

Primary human hepatocytes are an ideal source of hepatic function in bioartficial liver (BAL), but the shortage of human livers available for hepatocyte isolation limits this modality. To resolve this issue, primary human fetal hepatocytes were immortalized using simian virus 40 large T antigen. One...

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Published inCell transplantation Vol. 9; no. 5; pp. 733 - 735
Main Authors Kobayashi, Naoya, Miyazaki, Masahiro, Fukaya, Kenichi, Inoue, Yusuke, Sakaguchi, Masakiyo, Noguchi, Hirofumi, Matsumura, Toshihisa, Watanabe, Takamasa, Totsugawa, Toshinori, Tanaka, Noriaki, Namba, Masayoshi
Format Journal Article
LanguageEnglish
Published Los Angeles, CA SAGE Publications 01.09.2000
SAGE Publishing
Subjects
Ammonia - blood
Animals
Antigens, Polyomavirus Transforming - genetics
Cell Differentiation
Cell Line, Transformed
Cell Transformation, Viral
Hepatectomy
Hepatocytes - cytology
Hepatocytes - transplantation
Humans
Liver - embryology
Liver Failure, Acute - blood
Liver Failure, Acute - etiology
Liver Failure, Acute - surgery
Male
Rats
Rats, Sprague-Dawley
Spleen - surgery
Survival Rate
Acute liver failure
Hepatocyte transplantation
Simian virus 40 large T antigen
Immoralized human hepatocytes
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Summary:Primary human hepatocytes are an ideal source of hepatic function in bioartficial liver (BAL), but the shortage of human livers available for hepatocyte isolation limits this modality. To resolve this issue, primary human fetal hepatocytes were immortalized using simian virus 40 large T antigen. One of the immortal cell lines, OUMS-29, showed highly differentiated liver functions. Intrasplenic transplantation of OUMS-29 cells protected 90% hepatectomized rats from hyperammonemia and significantly prolonged their survival. Essentially unlimited availability of OUMS-29 cells supports their clinical use for BAL treatment.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0963-6897
1555-3892
DOI:10.1177/096368970000900524