Purification and Identification of a Nicotinic Acid-Replacing Substance from Vitamin-Free Acid Hydrolyzed Casein

A commercial preparation of vitamin free acid-hydrolyzed casein (casamino acids) contains a growth factor that substitutes for nicotinic acid in nicotinamide adenine dinucleotide auxotrophs of Escherichia coli. This replacing factor was purified by a combination of ethanol extraction, descending par...

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Bibliographic Details
Published inJournal of dairy science Vol. 66; no. 4; pp. 750 - 755
Main Authors Kerr, T.J., Williams, M.V., Tritz, G.J., Washam, C.J.
Format Journal Article
LanguageEnglish
Published Champaign Elsevier Inc 01.04.1983
Am Dairy Sci Assoc
American Dairy Science Association
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Summary:A commercial preparation of vitamin free acid-hydrolyzed casein (casamino acids) contains a growth factor that substitutes for nicotinic acid in nicotinamide adenine dinucleotide auxotrophs of Escherichia coli. This replacing factor was purified by a combination of ethanol extraction, descending partition chromatography, gel permeation chromatography, and crystallization. Structural analysis by mass spectrometry and infrared spectroscopy indicated that the compound has a mass of 368 daltons and contains six types of radicals. A possible structure is presented.
Bibliography:8269522
S10
ISSN:0022-0302
1525-3198
DOI:10.3168/jds.S0022-0302(83)81854-2