NADPH oxidase inhibitor diphenyleneiodonium and reduced glutathione mitigate ethephon-mediated leaf senescence, H2O2 elevation and senescence-associated gene expression in sweet potato (Ipomoea batatas)

• Published in: Journal of plant physiology Vol. 170; no. 17; pp. 1471 - 1483
• Main Authors: Chen, Hsien-Jung, Huang, Chin-Shu, Huang, Guan-Jhong, Chow, Te-Jin, Lin, Yaw-Huei
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 15.11.2013
• Subjects: Ascorbic Acid - metabolism; Buthionine Sulfoximine - pharmacology; Cellular Senescence; Chlorophyll - metabolism; Elevation; Ethephon; Ethylenes - metabolism; Gene expression; Gene Expression Regulation, Plant; Glutathione; Glutathione - metabolism; glutathione synthase; homeostasis; hydrogen peroxide; Hydrogen Peroxide - metabolism; Inhibitors; Ipomoea batatas; Ipomoea batatas - drug effects; Ipomoea batatas - genetics; Ipomoea batatas - metabolism; Leaf senescence; Leaves; NADP (coenzyme); NADPH oxidase; NADPH Oxidases - antagonists & inhibitors; Onium Compounds - pharmacology; Organophosphorus Compounds - pharmacology; Oxidase; oxidative stress; Plant Leaves - cytology; Plant Leaves - drug effects; Plant Leaves - metabolism; Reduced glutathione; senescence; Solanum tuberosum; Stresses; Sweet potato; sweet potatoes; Sweets; Reduced glutathione; DAB; Sweet potato; RT-PCR; NADPH oxidase; CCI; MES; Leaf senescence; DPI; Ethephon; diphenyleneiodonium; 2-(N-morpholino)-ethane sulphonic acid; chlorophyll content index; reverse transcription-polymerase chain reaction; diaminobenzidine

Ethephon, an ethylene releasing compound, promoted leaf senescence, H2O2 elevation, and senescence-associated gene expression in sweet potato. It also affected the glutathione and ascorbate levels, which in turn perturbed H2O2 homeostasis. The decrease of reduced glutathione and the accumulation of...