NADPH oxidase inhibitor diphenyleneiodonium and reduced glutathione mitigate ethephon-mediated leaf senescence, H2O2 elevation and senescence-associated gene expression in sweet potato (Ipomoea batatas)

• Published in: Journal of plant physiology Vol. 170; no. 17; pp. 1471 - 1483
• Main Authors: Chen, Hsien-Jung, Huang, Chin-Shu, Huang, Guan-Jhong, Chow, Te-Jin, Lin, Yaw-Huei
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 15.11.2013
• Subjects: Ascorbic Acid - metabolism; Buthionine Sulfoximine - pharmacology; Cellular Senescence; Chlorophyll - metabolism; Elevation; Ethephon; Ethylenes - metabolism; Gene expression; Gene Expression Regulation, Plant; Glutathione; Glutathione - metabolism; glutathione synthase; homeostasis; hydrogen peroxide; Hydrogen Peroxide - metabolism; Inhibitors; Ipomoea batatas; Ipomoea batatas - drug effects; Ipomoea batatas - genetics; Ipomoea batatas - metabolism; Leaf senescence; Leaves; NADP (coenzyme); NADPH oxidase; NADPH Oxidases - antagonists & inhibitors; Onium Compounds - pharmacology; Organophosphorus Compounds - pharmacology; Oxidase; oxidative stress; Plant Leaves - cytology; Plant Leaves - drug effects; Plant Leaves - metabolism; Reduced glutathione; senescence; Solanum tuberosum; Stresses; Sweet potato; sweet potatoes; Sweets; Reduced glutathione; DAB; Sweet potato; RT-PCR; NADPH oxidase; CCI; MES; Leaf senescence; DPI; Ethephon; diphenyleneiodonium; 2-(N-morpholino)-ethane sulphonic acid; chlorophyll content index; reverse transcription-polymerase chain reaction; diaminobenzidine
• ISSN: 0176-1617; 1618-1328
• DOI: 10.1016/j.jplph.2013.05.015

Ethephon, an ethylene releasing compound, promoted leaf senescence, H2O2 elevation, and senescence-associated gene expression in sweet potato. It also affected the glutathione and ascorbate levels, which in turn perturbed H2O2 homeostasis. The decrease of reduced glutathione and the accumulation of dehydroascorbate correlated with leaf senescence and H2O2 elevation at 72h in ethephon-treated leaves. Exogenous application of reduced glutathione caused quicker and significant increase of its intracellular level and resulted in the attenuation of leaf senescence and H2O2 elevation. A small H2O2 peak produced within the first 4h after ethephon application was also eliminated by reduced glutathione. Diphenyleneiodonium (DPI), an NADPH oxidase inhibitor, delayed leaf senescence and H2O2 elevation at 72h, and its influence was effective only within the first 4h after ethephon treatment. Ethephon-induced senescence-associated gene expression was repressed by DPI and reduced glutathione at 72h in pretreated leaves. Leaves treated with l-buthionine sulfoximine, an endogenous glutathione synthetase inhibitor, did enhance senescence-associated gene expression, and the activation was strongly repressed by reduced glutathione. In conclusion, ethephon-mediated leaf senescence, H2O2 elevation and senescence-associated gene expression are all alleviated by reduced glutathione and NADPH oxidase inhibitor DPI. The speed and the amount of intracellular reduced glutathione accumulation influence its effectiveness of protection against ethephon-mediated effects. Reactive oxygen species generated from NADPH oxidase likely serves as an oxidative stress signal and participates in ethephon signaling. The possible roles of NADPH oxidase and reduced glutathione in the regulation of oxidative stress signal in ethephon are discussed.