Roles of different active metal-reducing bacteria in arsenic release from arsenic-contaminated paddy soil amended with biochar

• Published in: Journal of hazardous materials Vol. 344; pp. 958 - 967
• Main Authors: Qiao, Jiang-tao, Li, Xiao-min, Li, Fang-bai
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.02.2018
• Subjects: Arsenic reduction; Biochar; Metal-reducing bacteria; Paddy soil; Transcriptional activity; Arsenic reduction; Biochar; Transcriptional activity; Metal-reducing bacteria; Paddy soil
• ISSN: 0304-3894; 1873-3336
• DOI: 10.1016/j.jhazmat.2017.11.025

[Display omitted] •Biochar amendment simultaneously increased microbial reduction of As(V) and Fe(III).•Biochar increased the abundance of Geobacter, Anaeromyxobacter and Clostridium.•The abundance of Geobacter transcripts closely tracked with As(V) contents.•The abundance of Clostridium transcripts closely tracked with Fe(III) contents.•Geobacter has a role in As(V) reduction while Clostridium was in Fe(III) reduction. Although biochar has great potential for heavy metal removal from sediments or soils, its impact on arsenic biogeochemistry in contaminated paddy fields remains poorly characterized. In this study, anaerobic microcosms were established with arsenic-contaminated paddy soil to investigate arsenic transformation as well as the potentially active microbial community and their transcriptional activities in the presence of biochar. The results demonstrated that biochar can simultaneously stimulate microbial reduction of As(V) and Fe(III), releasing high levels of As(III) into the soil solution relative to the control. Total RNAs were extracted to profile the potentially active microbial communities, which suggested that biochar increased the abundance of arsenic- and iron-related bacteria, such as Geobacter, Anaeromyxobacter and Clostridium compared to the control. Reverse transcription, quantitative PCR (RT-qPCR) showed that the abundance of Geobacter transcripts were significantly stimulated by biochar throughout the incubation. Furthermore, significant positive correlations were observed between the abundance of Geobacter transcripts and As(V) concentrations, and between that of Clostridium transcripts and Fe(III) concentrations in biochar-amended microcosms. Our findings suggest that biochar can stimulate the activity of metal-reducing bacteria to promote arsenic mobility. The Geobacter may contribute to As(V) reduction in the presence of biochar, while Clostridium has a role in Fe(III) reduction.