Expression and function of miRNA in postoperative radiotherapy sensitive and resistant patients of non-small cell lung cancer

• Published in: Lung cancer (Amsterdam, Netherlands) Vol. 72; no. 1; pp. 92 - 99
• Main Authors: Wang, Xiao-Chun, Du, Li-Qing, Tian, Li-Li, Wu, Hai-Liang, Jiang, Xiao-Yan, Zhang, Heng, Li, De-Guan, Wang, Yue-Ying, Wu, Hong-Ying, She, Yi, Liu, Qing-fen, Fan, Fei-Yue, Meng, Ai-Min
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ireland Ltd 01.04.2011; Elsevier
• Subjects: Adult; Aged; Apoptosis; Apoptosis - genetics; Apoptosis - radiation effects; Biological and medical sciences; Carcinoma, Non-Small-Cell Lung - genetics; Carcinoma, Non-Small-Cell Lung - metabolism; Carcinoma, Non-Small-Cell Lung - radiotherapy; Carcinoma, Non-Small-Cell Lung - surgery; Cell Line, Tumor; Cell Proliferation - radiation effects; Cell Survival - genetics; Cell Survival - radiation effects; Female; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Hematology, Oncology and Palliative Medicine; Humans; Lung Neoplasms - genetics; Lung Neoplasms - metabolism; Lung Neoplasms - radiotherapy; Lung Neoplasms - surgery; Male; Medical sciences; MicroRNAs - genetics; MicroRNAs - metabolism; Middle Aged; miRNA; NSCLC; Oncogene Protein v-akt - metabolism; Phosphatidylinositol 3-Kinases - metabolism; Pneumology; Postoperative Period; Pulmonary/Respiratory; Radiation Tolerance; Radiotherapy sensitivity; Signal Transduction; Tumors; Tumors of the respiratory system and mediastinum; miRNA; Radiotherapy sensitivity; NSCLC; Apoptosis; Human; Postoperative; Lung disease; Respiratory disease; Malignant tumor; non-small cell lung carcinoma; Gene expression; Radiotherapy; Sensitivity; Cancerology; Treatment; Bronchus disease; Cancer; Non small cell carcinoma; Pneumology
• ISSN: 0169-5002; 1872-8332
• DOI: 10.1016/j.lungcan.2010.07.014

Abstract Purpose To investigate the different miRNA expression profiles of postoperative radiotherapy sensitive and resistant patients of non-small cell lung cancer, explore their potential role and find some radio-sensitivity markers. Materials and methods Thirty non-small cell lung cancer patients who have been treated by postoperative radiotherapy were selected and were divided into radiotherapy sensitive group and resistant group according to overall survival and local or distant recurrence rate. Expression profile of miRNA in these two groups was detected by a microarray assay and the results were validated by quantitative RT-PCR and Northern blot. At the molecular level, the effect of one differently expressed miRNA (miR-126) on the growth and apoptosis of SK-MES-1 cells induced by irradiation was examined. Results Comparing with resistant patients, five miRNAs (miRNA-126, miRNA-let-7a, miRNA-495, miRNA-451 and miRNA-128b) were significantly upregulated and seven miRNAs (miRNA-130a, miRNA-106b, miRNA-19b, miRNA-22, miRNA-15b, miRNA-17-5p and miRNA-21) were greatly downregulated in radiotherapy sensitive group. Overexpression of miRNA-126 inhibited the growth of SK-MES-1 cells and promoted its apoptosis induced by irradiation. The expression level of p-Akt decreased in miRNA-126 overexpression group. After treating with phosphoinositidyl-3 kinase (PI3K) constitutively activator (IGF-1) and inhibitor (LY294002), miRNA-126 overexpression had no significant effects on the apoptosis of SK-MES-1 cells. Conclusion We found 12 differently expressed miRNAs in the radiotherapy sensitive and resistant non-small cell lung cancer samples. Moreover, our results showed miRNA-126 promoted non-small cell lung cancer cells apoptosis induced by irradiation through the PI3K-Akt pathway.