Cyanidin-3-O-Galactoside-Enriched Aronia melanocarpa Extract Attenuates Weight Gain and Adipogenic Pathways in High-Fat Diet-Induced Obese C57BL/6 Mice

• Published in: Nutrients Vol. 11; no. 5; p. 1190
• Main Authors: Lim, Su-Min, Lee, Hyun Sook, Jung, Jae In, Kim, So Mi, Kim, Nam Young, Seo, Tae Su, Bae, Jung-Shik, Kim, Eun Ji
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.05.2019; MDPI
• Subjects: adipogenesis; adipogenic transcription factor; Animal models; Antioxidants; Aronia melanocarpa; Atherosclerosis; Body fat; Body weight; Cardiovascular disease; Cardiovascular diseases; cyanidin-3-O-galactoside; Diabetes; Diabetes mellitus; Diet; Disease resistance; Fatty acids; Galactosides; High fat diet; high fat induced obesity; Hypertension; Insulin; Insulin resistance; Kinases; Medical treatment; Metabolic disorders; Metabolic syndrome; Obesity; Oxidation resistance; Oxidative stress; Physiological effects; Physiology; Plant diseases; Polyphenols; Proteins; Transcription factors; United States > US
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu11051190

Aronia melanocarpa are a rich source of anthocyanins that have received considerable interest for their relations to human health. In this study, the anti-adipogenic effect of cyanidin-3-O-galactoside-enriched Aronia melanocarpa extract (AM-Ex) and its underlying mechanisms were investigated in an in vivo system. Five-week-old male C57BL/6N mice were randomly divided into five groups for 8-week feeding with a control diet (CD), a high-fat diet (HFD), or a HFD with 50 (AM-Ex 50), 100 (AM-Ex 100), or 200 AM-Ex (AM-Ex 200) mg/kg body weight/day. HFD-fed mice showed a significant increase in body weight compared to the CD group, and AM-Ex dose-dependently inhibited this weight gain. AM-Ex significantly reduced the food intake and the weight of white fat tissue, including epididymal fat, retroperitoneal fat, mesenteric fat, and inguinal fat. Treatment with AM-Ex (50 to 200 mg/kg) reduced serum levels of leptin, insulin, triglyceride, total cholesterol, and low density lipoprotein (LDL)-cholesterol. Real-time reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed that AM-Ex suppressed adipogenesis by decreasing CCAAT/enhancer binding protein α, peroxisome proliferator-activated receptor γ, sterol regulatory element-binding protein-1c, peroxisome proliferator-activated receptor gamma coactivator-1α, acetyl-CoA carboxylase 1, ATP-citrate lyase, fatty acid synthase, and adipocyte protein 2 messenger RNA (mRNA) expressions. These results suggest that AM-Ex is potentially beneficial for the suppression of HFD-induced obesity by modulating multiple pathways associated with adipogenesis and food intake.