PDGFRβ Activation Induced the Bovine Embryonic Genome Activation via Enhanced NFYA Nuclear Localization

• Published in: International journal of molecular sciences Vol. 24; no. 23; p. 17047
• Main Authors: Perera, Chalani Dilshani, Idrees, Muhammad, Khan, Abdul Majid, Haider, Zaheer, Ullah, Safeer, Kang, Ji-Su, Lee, Seo-Hyun, Kang, Seon-Min, Kong, Il-Keun
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.12.2023
• Subjects: activator; Animals; Becaplermin - metabolism; bovine blastocysts; Cattle; Cell cycle; Cell Differentiation; Cell division; DNA binding proteins; Embryonic development; embryonic genome activation; Embryos; Genes; Genetic transcription; Genomes; Genomics; Kinases; Localization; NFYA; PDGFRβ; Platelet-derived growth factor; Proteins; Receptor, Platelet-Derived Growth Factor alpha - genetics; Receptor, Platelet-Derived Growth Factor alpha - metabolism; Receptor, Platelet-Derived Growth Factor beta - genetics; Receptor, Platelet-Derived Growth Factor beta - metabolism; RNA; Signal Transduction - physiology; Transcription factors; PDGFRβ; activator; embryonic genome activation; NFYA; bovine blastocysts
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms242317047

Embryonic genome activation (EGA) is a critical step during embryonic development. Several transcription factors have been identified that play major roles in initiating EGA; however, this gradual and complex mechanism still needs to be explored. In this study, we investigated the role of nuclear transcription factor Y subunit A (NFYA) in bovine EGA and bovine embryonic development and its relationship with the platelet-derived growth factor receptor-β (PDGFRβ) by using a potent selective activator (PDGF-BB) and inhibitor (CP-673451) of PDGF receptors. Activation and inhibition of PDGFRβ using PDGF-BB and CP-673451 revealed that NFYA expression is significantly ( 0.05) affected by the PDGFRβ. In addition, PDGFRβ mRNA expression was significantly increased ( 0.05) in the activator group and significantly decreased ( 0.05) in the inhibitor group when compared with PDGFRα. Downregulation of NFYA following PDGFRβ inhibition was associated with the expression of critical EGA-related genes, bovine embryo development rate, and implantation potential. Moreover, ROS and mitochondrial apoptosis levels and expression of pluripotency-related markers necessary for inner cell mass development were also significantly ( 0.05) affected by the downregulation of NFYA while interrupting trophoblast cell ( ) differentiation. In conclusion, the PDGFRβ-NFYA axis is critical for bovine embryonic genome activation and embryonic development.