Optimize, Modulate, and Scale-up Resveratrol and Resveratrol Dimers Bioproduction in Vitis labrusca L. Cell Suspension from Flasks to 20 L Bioreactor

• Published in: Plants (Basel) Vol. 8; no. 12; p. 567
• Main Authors: Lambert, Carole, Lemaire, Julien, Auger, Hélène, Guilleret, Arnaud, Reynaud, Romain, Clément, Christophe, Courot, Eric, Taidi, Behnam
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 04.12.2019; MDPI
• Subjects: Bioactive compounds; Biological activity; Biomass; Bioreactors; Biotechnology; Cancer; Cell culture; Chemical and Process Engineering; Cyclodextrin; Cyclodextrins; Dimers; elicitation; Engineering Sciences; Flasks; Growth rate; Life Sciences; Methyl jasmonate; methyl > cyclodextrin; Oligomers; plant cell culture; Resveratrol; Statistical analysis; stilbene; Sucrose; Variance analysis; Vegetal Biology; viniferins; Vitis labrusca; elicitation; methyl-β-cyclodextrin; viniferins; stilbene; methyl jasmonate; plant cell culture; bioreactors; Vitis labrusca; resveratrol
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants8120567

Resveratrol and its oligomers are biologically active compounds. This work brings new insights for the bioproduction of -resveratrol with three dimers, pallidol, -ε-viniferin, and -δ-viniferin, in cell suspension of . Conditions of elicitation by methyl jasmonate were optimized for the production of stilbenes using statistical design of experiment. Bio-production of stilbenes was scaled-up to 5 L and in these conditions, -resveratrol concentrations reached 237 mg/L, and for pallidol 114 mg/L. The comparison of different elicitation modes (different elicitors, combination with cyclodextrins or adsorbent resin) allowed to reach particularly high concentrations of target molecules: Resveratrol 6.14 g/L, pallidol 0.90 g/L, δ-viniferin 0.54 g/L, and ε-viniferin 0.50 g/L. Scale-up to 20 L-stirring-bioreactor gave similar growth rates to those observed in shake flask culture, with a high production of resveratrol (4.23 g/L) and δ-viniferin (0.76 g/L). This work provides new strategies for the production of stilbenes in plant cell suspension for biological and commercial evaluation.