Genetic recombination in sexual crosses of Phycomyces [blakesleeanus, fungi]
Sexual crosses between strains of Phycomyces blakesleeanus , involving three auxotrophic and one color marker and yielding a high proportion of zygospore germination, are described. Samples of 20-40 germ spores from 311 individual fertile germ sporangia originating from five two-factor and three thr...
Saved in:
Published in | Genetics (Austin) Vol. 80; no. 3; pp. 445 - 462 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
United States
Genetics Soc America
01.07.1975
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Sexual crosses between strains of Phycomyces blakesleeanus , involving three auxotrophic and one color marker and yielding a high proportion of zygospore germination, are described. Samples of 20-40 germ spores from 311 individual fertile germ sporangia originating from five two-factor and three three-factor crosses were characterized. The results show: (1) absence of any contribution of apogamic nuclei to the progeny, (2) confirmation of Burgeff's conjecture that the germ spores of any germ sporangium in most cases derive from one meiosis. In a cross involving two allelic markers the analysis of 175 pooled germ sporangia suggests an intragenic recombination frequency of 0.6%. All other factor combinations tested are unlinked. The bulk of the germ spores are homokaryotic. However, a small portion (4%) are heterokaryotic with respect to mating type. |
---|---|
Bibliography: | F30 F ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0016-6731 1943-2631 1943-2631 |
DOI: | 10.1093/genetics/80.3.445 |