Analysis of the laccase gene family and miR397-/miR408-mediated posttranscriptional regulation in Salvia miltiorrhiza
Salvia miltiorrhiza is one of the most commonly used traditional Chinese medicine materials. It contains important bioactive phenolic compounds, such as salvianolic acids, flavonoids and anthocyanins. Elucidation of phenolic compound biosynthesis and its regulatory mechanism is of great significance...
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Published in | PeerJ (San Francisco, CA) Vol. 7; p. e7605 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
San Diego
PeerJ, Inc
29.08.2019
PeerJ Inc |
Subjects | |
Online Access | Get full text |
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Summary: | Salvia miltiorrhiza
is one of the most commonly used traditional Chinese medicine materials. It contains important bioactive phenolic compounds, such as salvianolic acids, flavonoids and anthocyanins. Elucidation of phenolic compound biosynthesis and its regulatory mechanism is of great significance for
S. miltiorrhiza
quality improvement. Laccases (LACs) are multicopper-containing enzymes potentially involved in the polymerization of phenolic compounds. So far, little has been known about
LAC
genes in
S. miltiorrhiza
. Through systematic investigation of the whole genome sequence and transcriptomes of
S. miltiorrhiza
, we identified 65 full-length
SmLAC
genes (
SmLAC1
–
SmLAC65
). Phylogenetic analysis showed that 62 of the identified SmLACs clustered with LACs from
Arabidopsis
and
Populus trichocarpa
in seven clades (C1–C7), whereas the other three fell into one
S. miltiorrhiza
-specific clade (C8). All of the deduced SmLAC proteins contain four conserved signature sequences and three typical Cu-oxidase domains, and gene structures of most
LACs
from
S. miltiorrhiza
,
Arabidopsis
and
P. trichocarpa
were highly conserved, however
SmLACs
encoding C8 proteins showed distinct intron-exon structures. It suggests the conservation and diversity of plant
LACs
in gene structures. The majority of
SmLACs
exhibited tissue-specific expression patterns, indicates manifold functions of
SmLACs
played in
S. miltiorrhiza
. Analysis of high-throughput small RNA sequences and degradome data and experimental validation using the 5′ RACE method showed that 23
SmLACs
were targets of Smi-miR397. Among them, three were also targeted by Smi-miR408. It suggests the significance of miR397 and miR408 in posttranscriptional regulation of
SmLAC
genes. Our results provide a foundation for further demonstrating the functions of
SmLACs
in the production of bioactive phenolic compounds in
S. miltiorrhiza
. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2167-8359 2167-8359 |
DOI: | 10.7717/peerj.7605 |