Trophic effect of erythropoietin and other hematopoietic factors on central cholinergic neurons in vitro and in vivo

• Published in: Brain research Vol. 609; no. 1; pp. 29 - 35
• Main Authors: Konishi, Yoshihiro, Chui, De-Hua, Hirose, Hideki, Kunishita, Tatsuhide, Tabira, Takeshi
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 23.04.1993; Amsterdam Elsevier; New York, NY
• Subjects: Animals; Biological and medical sciences; Cell Line; Cells, Cultured; Choline O-Acetyltransferase - metabolism; Cholinergic neuron; Erythroid differentiation factor; Erythropoietin; Erythropoietin - pharmacology; Fundamental and applied biological sciences. Psychology; Granulocyte colony-stimulating factor; Granulocyte Colony-Stimulating Factor - physiology; Hematopoiesis - drug effects; Hematopoietic factor; Hippocampus - physiology; Humans; Isolated neuron and nerve. Neuroglia; Macrophage colony-stimulating factor; Macrophage Colony-Stimulating Factor - physiology; Male; Mice; Mice, Inbred BALB C; Neurons - drug effects; Neurotrophic factor; Parasympathetic Nervous System - cytology; Parasympathetic Nervous System - drug effects; Rats; Rats, Wistar; Receptor, Macrophage Colony-Stimulating Factor; Receptors, Granulocyte Colony-Stimulating Factor; Recombinant Proteins - pharmacology; Vertebrates: nervous system and sense organs; Neurotrophic factor; Cholinergic neuron; Hematopoietic factor; Granulocyte colony-stimulating factor; Erythropoietin; Macrophage colony-stimulating factor; Erythroid differentiation factor; Rat; Enzyme; Rodentia; Central nervous system; Trophic factor; Choline acetyltransferase; Regeneration; Vertebrata; Granulocyte colony stimulating factor; Mammalia; Cell line; Enzymatic activity; Mouse; Development; Macrophage colony stimulating factor; Differentiation; Brain (vertebrata)
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/0006-8993(93)90850-M

In vitro granulocyte colony-stimulating factor (G-CSF), macrophage colony-stimulating factor (M-CSF), erythropoietin (EPO), and erythroid differentiation factor (EDF) augmented choline acetyltransferase (ChAT) activity in mouse embryonic primary septal neurons and in cholinergic hybridoma cell line, SN6.10.2.2. This is similar to the effects seen with interleukin-3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF). Moreover, in vivo GM-CSF and EPO promoted survival of septal cholinergic neurons in adult rats which had undergone fimbria-fornix transections. These results suggest that some of the hematopoietic factors act on cholinergic neurons as ‘neurotrophic factors’ to influence the differentiation, maintenance and regeneration of these neurons.