Cytogenetic and Molecular Aspects of Absolute Teratozoospermia: Comparison Between Polymorphic and Monomorphic Forms

• Published in: Urology (Ridgewood, N.J.) Vol. 78; no. 6; pp. 1313 - 1319
• Main Authors: Brahem, Sonia, Elghezal, Hatem, Ghédir, Houda, Landolsi, Hanène, Amara, Abdelbacett, Ibala, Samira, Gribaa, Moez, Saad, Ali, Mehdi, Meriem
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.12.2011; Elsevier
• Subjects: Adult; Aneuploidy; Biological and medical sciences; Chromosomes, Human, Y; DNA Fragmentation; Humans; In Situ Hybridization, Fluorescence; In Situ Nick-End Labeling; Infertility, Male - genetics; Infertility, Male - pathology; Karyotype; Male; Medical sciences; Middle Aged; Nephrology. Urinary tract diseases; Spermatozoa - pathology; Urology; Cytogenetics; Nephrology; Comparative study; Urology; Polymorphism
• ISSN: 0090-4295; 1527-9995
• DOI: 10.1016/j.urology.2011.08.064

Objective To compare the results of cytogenetic and molecular analysis between absolute polymorphic and monomorphic teratozoospermia. Methods The semen samples from patients with polymorphic teratozoospermia (n = 20), globozoospermia (n = 8), or macrocephalic sperm head syndrome (n = 12), and healthy fertile men (n = 20) were analyzed according to the World Health Organization criteria. The constitutional blood karyotype of the patients was performed on cultured lymphocytes, according to standard techniques. Microdeletion analysis of the Y chromosomes used a sequence tagged site-polymerase chain reaction technique. Triple-color fluorescent in situ hybridization for chromosomes X, Y, and 18 were used to analyze the meiotic segregation. DNA fragmentation was detected using the terminal desoxynucleotidyl transferase-mediated deoxyuridine triphosphate biotin nick-end labeling assay. Results Whatever the type of teratozoospermia, a normal karyotype and an absence of Y chromosome microdeletion were shown for all patients. A significant increase in the sperm aneuploidy rate and DNA fragmentation were shown, regardless of the type of teratozoospermia. Spermatozoa of the patients with globozoospermia carry an abnormal chromosomal constitution and DNA damage rate with the same frequency as that found in the sperm of patients with absolute polymorphic teratozoospermia. However, a greater sperm aneuploidy rate and DNA fragmentation were found in patients whose teratozoospermia was mainly characterized by increased rates of spermatozoa with macrocephalic head and multiple flagella. Conclusion Our data have demonstrated that DNA fragmentation and sperm aneuploidy are critical tests in teratozoospermic men, because the results could negatively affect the intracytoplasmic sperm injection outcomes and might play an important role in the counseling of couples considering intracytoplasmic sperm injection.