L -cysteine transporter-PCR to detect hydrogen sulfide-producing Campylobacter fetus

• Published in: PeerJ (San Francisco, CA) Vol. 7; p. e7820
• Main Authors: Farace, Pablo D., Morsella, Claudia G., Cravero, Silvio L., Sioya, Bernardo A., Amadio, Ariel F., Paolicchi, Fernando A., Gioffré, Andrea K.
• Format: Journal Article
• Language: English
• Published: San Diego PeerJ, Inc 05.11.2019; PeerJ Inc
• Subjects: Agricultural Science; Bioinformatics; Bovine genital campylobacteriosis; Campylobacter; Campylobacter fetus; Campylobacteriosis; Cysteine; Deoxyribonucleic acid; Differential diagnosis; DNA; Fetuses; Gene deletion; Genes; Genomes; Hydrogen sulfide; Molecular Biology; Molecular differentiation; PCR; Proteins; Sulfide production; United Kingdom > UK; United States > US; Argentina
• ISSN: 2167-8359; 2167-8359
• DOI: 10.7717/peerj.7820

Phenotypic differences between Campylobacter fetus fetus and C. fetus venerealis subspecies allow the differential diagnosis of bovine genital campylobacteriosis. The hydrogen sulfide production, for example, is a trait exclusive to C. fetus fetus and C. fetus venerealis biovar intermedius. This gas that can be biochemically tested can be produced from L -cysteine ( L -Cys). Herein, we report a novel multiplex-PCR to differentiate C. fetus based on the evaluation of a deletion of an ATP-binding cassette-type L -Cys transporter that could be involved in hydrogen sulfide production, as previously described. A wet lab approach combined with an in silico whole genome data analysis showed complete agreement between this L -Cys transporter-PCR and the hydrogen sulfide production biochemical test. This multiplex-PCR may complement the tests currently employed for the differential diagnosis of C. fetus .