Small molecule based fluorescent chemosensors for imaging the microenvironment within specific cellular regions
The microenvironment (local environment), including viscosity, temperature, polarity, hypoxia, and acidic-basic status (pH), plays indispensable roles in cellular processes. Significantly, organelles require an appropriate microenvironment to perform their specific physiological functions, and disru...
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| Published in | Chemical Society reviews Vol. 5; no. 21; pp. 1298 - 1215 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
London
Royal Society of Chemistry
01.11.2021
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| Subjects | |
| Online Access | Get full text |
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| Summary: | The microenvironment (local environment), including viscosity, temperature, polarity, hypoxia, and acidic-basic status (pH), plays indispensable roles in cellular processes. Significantly, organelles require an appropriate microenvironment to perform their specific physiological functions, and disruption of the microenvironmental homeostasis could lead to malfunctions of organelles, resulting in disorder and disease development. Consequently, monitoring the microenvironment within specific organelles is vital to understand organelle-related physiopathology. Over the past few years, many fluorescent probes have been developed to help reveal variations in the microenvironment within specific cellular regions. Given that a comprehensive understanding of the microenvironment in a particular cellular region is of great significance for further exploration of life events, a thorough summary of this topic is urgently required. However, there has not been a comprehensive and critical review published recently on small-molecule fluorescent chemosensors for the cellular microenvironment. With this review, we summarize the recent progress since 2015 towards small-molecule based fluorescent probes for imaging the microenvironment within specific cellular regions, including the mitochondria, lysosomes, lipid drops, endoplasmic reticulum, golgi, nucleus, cytoplasmic matrix and cell membrane. Further classifications at the suborganelle level, according to detection of microenvironmental factors by probes, including polarity, viscosity, temperature, pH and hypoxia, are presented. Notably, in each category, design principles, chemical synthesis, recognition mechanism, fluorescent signals, and bio-imaging applications are summarized and compared. In addition, the limitations of the current microenvironment-sensitive probes are analyzed and the prospects for future developments are outlined. In a nutshell, this review comprehensively summarizes and highlights recent progress towards small molecule based fluorescent probes for sensing and imaging the microenvironment within specific cellular regions since 2015. We anticipate that this summary will facilitate a deeper understanding of the topic and encourage research directed towards the development of probes for the detection of cellular microenvironments.
This review comprehensively summarizes and highlights recent progresses in the design and application of small molecule based fluorescent probes for sensing and imaging the microenvironment within specific cellular regions since 2015. |
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| Bibliography: | Jiangfeng Li obtained his bachelor's degree from Shaanxi University of Science & Technology in 2016. Currently, he is a doctoral student under the supervision of Professor Weiying Lin in the college of chemistry and chemical engineering, Guangxi University. His research interests focus on the design and synthesis of novel functional fluorescent dyes/probes and their biological applications. Weiying Lin received his PhD from the University of Kansas in 2000. After completing postdoctoral research at Massachusetts Institute of Technology, in 2005, he joined the faculty at Hunan University. Subsequently, he moved to the University of Jinan as the Dean and the Distinguished Professor of the Institute of Fluorescent Probes for Biological Imaging. Now, he is a professor at the Guangxi University. His research interests cover the interdisciplinary areas of molecular recognition, photochemistry, analytical chemistry, and chemical biology. His research group has published over 350 papers in the area of fluorescent probes for biological imaging. Ling Huang obtained her bachelor's degree from Yulin Normal University in 2016. Currently, she is a doctoral student under the supervision of Professor Weiying Lin in the college of chemistry and chemical engineering, Guangxi University. Her research interests focus on the design, synthesis and biological applications of novel functional fluorescent dyes/probes. Luling Wu received his MSc in 2017 from Shanghai Normal University. In 2017, he was awarded scholarships by the China Scholarship Council (CSC) and University of Bath to carry out a PhD at the University of Bath. He is a guest editor of a special issue on "Molecular Sensors and Molecular Logic Gates" for Frontiers of Chemical Science and Engineering (FCSE), and referee for Cell Reports. He is an editor for "Fluorescent Chemosensors" as part of the Monographs in Supramolecular Chemistry published by the Royal Society of Chemistry. Dr Junling Yin received her PhD degree from the University of Jinan in 2021 under the supervision of Professor Weiying Lin. She is currently working in the Science and Technology Innovation Center, Shandong First Medical University & Shandong Academy of Medical Sciences. Her research interests focus on the design and synthesis of novel probes for the detection of biological microenvironments. Tony D. James is a Professor at the University of Bath, Fellow of the Royal Society of Chemistry and holds a prestigious Royal Society Wolfson Research Merit Award (2017-2022). He obtained his BSc from the University of East Anglia (1986), PhD from the University of Victoria (1991), and carried out Postdoctoral Research with Seiji Shinkai (1991-95). He was a Royal Society University Research Fellow at the University of Birmingham (1995-2000) before moving to the University of Bath in 2001. In 2013 he was awarded the Daiwa-Adrian Prize, in 2015 the inaugural CASE Prize, 2018 the MSMLG Czarnik Award and 2020 Frontiers in Chemistry Diversity Award. His research interests include many aspects of Supramolecular chemistry, including molecular recognition, molecular self-assembly and sensor design. He is the author of over 355 papers and has an h-index of 74. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 ObjectType-Review-3 content type line 23 |
| ISSN: | 0306-0012 1460-4744 1460-4744 |
| DOI: | 10.1039/d1cs00645b |