Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta as a potential biomarker for Opisthorchis viverrini infection and cholangiocarcinoma

• Published in: Parasitology Vol. 149; no. 2; pp. 171 - 180
• Main Authors: Prasopdee, Sattrachai, Yingchutrakul, Yodying, Roytrakul, Sittiruk, Pholhelm, Montinee, Phanaksri, Teva, Kunjantarachot, Anthicha, Kulsantiwong, Jutharat, Butthongkomvong, Kritiya, Tesana, Smarn, Sathavornmanee, Thanakrit, Thitapakorn, Veerachai
• Format: Journal Article
• Language: English
• Published: Cambridge, UK Cambridge University Press 01.02.2022
• Subjects: AKT protein; Amino acids; Animals; Apoptosis; Bile Duct Neoplasms - diagnosis; Bile Duct Neoplasms - epidemiology; Bile Duct Neoplasms - metabolism; Bile ducts; Bile Ducts, Intrahepatic - metabolism; Bile Ducts, Intrahepatic - parasitology; Bile Ducts, Intrahepatic - pathology; Biomarkers; Biomarkers - analysis; Catalytic Domain; Cell cycle; Cell growth; Cholangiocarcinoma; Cholangiocarcinoma - diagnosis; Cholangiocarcinoma - etiology; Cholangiocarcinoma - metabolism; early diagnosis; Enzyme-linked immunosorbent assay; Humans; Infections; Kinases; Liver; liver flukes; opisthorchiasis; Opisthorchiasis - diagnosis; Opisthorchiasis - epidemiology; Opisthorchiasis - parasitology; Opisthorchis; Opisthorchis viverrini; Parasites; parasitology; Peptides; Phosphatidylinositol 4,5-diphosphate; Phosphatidylinositols - metabolism; phosphoproteome; Phosphoproteomes; Plasma; protein subunits; Proteins; Public health; Risk analysis; Risk factors; Sensitivity; Signal transduction; Signaling; Ultrasonic imaging; Thailand; United States > US; diagnosis; Biomarker; Phosphoproteome; PIK3CB; cholangiocarcinoma; Opisthorchis viverrini
• ISSN: 0031-1820; 1469-8161; 1469-8161
• DOI: 10.1017/S0031182021001694

The human liver fluke Opisthorchis viverrini (Ov), the primary risk factor for cholangiocarcinoma (CHCA), is a parasite endemic to southeast Asian countries. With no effective treatments for CHCA currently available, early diagnosis and treatment of Ov infection remains the only practical method for the prevention of CHCA. In this study, plasma phosphoproteomes of patients in the non-Ov infection, non-cholangiocarcinoma subject group (non-OVCCA), the asymptomatic Ov infected group (OV), and the CHCA group (CCA), were investigated to identify potential biomarkers for Ov infection and CHCA. The AKT signalling pathway was found to be up-regulated. Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform (PIK3CB), an upstream signalling molecule, was selected as a potential biomarker and evaluated using indirect enzyme-linked immunosorbent assay (ELISA). Results demonstrated evidence that levels of PIK3CB in both the OV group and CCA group was statistically different compared to the non-OVCCA group (P < 0.01). However, the levels of PIK3CB between the OV group and the CCA group were found not to be statistically different. Sensitivity and specificity for OV using OD450 cut-off at >1.570 was 76 and 72%, respectively. For CCA, sensitivity and specificity using OD450 cut-off at >1.398 was 68 and 76%, respectively. Application of indirect ELISA detecting plasma PIK3CB will be of great benefit for screening of opisthorchiasis and CHCA.