Cardiac troponin T N-domain variant destabilizes the actin interface resulting in disturbed myofilament function

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 120; no. 23; p. e2221244120
• Main Authors: Landim-Vieira, Maicon, Ma, Weikang, Song, Taejeong, Rastegarpouyani, Hosna, Gong, Henry, Coscarella, Isabella Leite, Bogaards, Sylvia J P, Conijn, Stefan P, Ottenheijm, Coen A C, Hwang, Hyun S, Papadaki, Maria, Knollmann, Bjorn C, Sadayappan, Sakthivel, Irving, Thomas C, Galkin, Vitold E, Chase, P Bryant, Pinto, Jose Renato
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 06.06.2023; Proceedings of the National Academy of Sciences
• Subjects: Actin; Actin Cytoskeleton - genetics; Actins - genetics; Actomyosin; Biological Sciences; Calcium; Calcium ions; Cardiomyopathy; Destabilization; Humans; Hydrophobicity; Muscles; Mutation; Myofibrils - genetics; Myofibrils - pathology; Myosin; Myosins; Pathogenesis; Stiffness; Troponin; Troponin T; Troponin T - chemistry; Troponin T - genetics; cardiac thin filament; TnT1 loop region; myosin SRX/DRX; cardiac troponin T tail domain; cardiomyopathy
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.2221244120

Missense variant Ile79Asn in human cardiac troponin T (cTnT-I79N) has been associated with hypertrophic cardiomyopathy and sudden cardiac arrest in juveniles. cTnT-I79N is located in the cTnT N-terminal (TnT1) loop region and is known for its pathological and prognostic relevance. A recent structural study revealed that I79 is part of a hydrophobic interface between the TnT1 loop and actin, which stabilizes the relaxed (OFF) state of the cardiac thin filament. Given the importance of understanding the role of TnT1 loop region in Ca regulation of the cardiac thin filament along with the underlying mechanisms of cTnT-I79N-linked pathogenesis, we investigated the effects of cTnT-I79N on cardiac myofilament function. Transgenic I79N (Tg-I79N) muscle bundles displayed increased myofilament Ca sensitivity, smaller myofilament lattice spacing, and slower crossbridge kinetics. These findings can be attributed to destabilization of the cardiac thin filament's relaxed state resulting in an increased number of crossbridges during Ca activation. Additionally, in the low Ca -relaxed state (pCa8), we showed that more myosin heads are in the disordered-relaxed state (DRX) that are more likely to interact with actin in cTnT-I79N muscle bundles. Dysregulation of the myosin super-relaxed state (SRX) and the SRX/DRX equilibrium in cTnT-I79N muscle bundles likely result in increased mobility of myosin heads at pCa8, enhanced actomyosin interactions as evidenced by increased active force at low Ca , and increased sinusoidal stiffness. These findings point to a mechanism whereby cTnT-I79N weakens the interaction of the TnT1 loop with the actin filament, which in turn destabilizes the relaxed state of the cardiac thin filament.