Transcriptome analysis of differential gene expression in Dichomitus squalens during interspecific mycelial interactions and the potential link with laccase induction

• Published in: The journal of microbiology Vol. 57; no. 2; pp. 127 - 137
• Main Authors: Zhong, Zixuan, Li, Nannan, He, Binghui, Igarashi, Yasuo, Luo, Feng
• Format: Journal Article
• Language: English
• Published: Seoul The Microbiological Society of Korea 01.02.2019; Springer Nature B.V; 한국미생물학회
• Subjects: Alcohols; Biomedical and Life Sciences; Chitinase; Cytochrome P450; Cytochromes; Cytochromes P450; Detoxification; DNA; Fungi; Fungicides; Gene expression; Genes; Genomics and Molecular Biology; Glutathione; Glycoside hydrolase; Glycosides; Hydrolase; Interactions; Interspecific; Laccase; Life Sciences; Manganese; Manganese peroxidase; Microbial Genetics; Microbiology; Mineral nutrients; Mycelia; Nucleotide sequence; Nutrients; Oxidative stress; PCR; Peroxidase; Proteins; Reactive oxygen species; Reductase; Transferases; White rot; White rot fungi; 생물학; mycelial interactions; white rot fungi; transcriptome analysis; laccase; Dichomitus squalens
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-019-8398-y

Interspecific mycelial interactions between white rot fungi are always accompanied by an increased production of laccase. In this study, the potential of the white rot fungus Dichomitus squalens to enhance laccase production during interactions with two other white rot fungi, Trametes versicolor or Pleurotus ostreatus , was assessed. To probe the mechanism of laccase induction and the role that laccase plays during combative interaction, we analyzed the differential gene expression profile of the laccase induction response to stressful conditions during fungal interaction. We further confirmed the expression patterns of 16 selected genes by qRT-PCR analysis. We noted that many differentially expressed genes (DEGs) encoded proteins that were involved in xenobiotic detoxification and reactive oxygen species (ROS) generation or reduction, including aldo/keto reductase, glutathione S-transferases, cytochrome P450 enzymes, alcohol oxidases and dehydrogenase, manganese peroxidase and laccase. Furthermore, many DEG-encoded proteins were involved in antagonistic mechanisms of nutrient acquisition and antifungal properties, including glycoside hydrolase, glucanase, chitinase and terpenoid synthases. DEG analyses effectively revealed that laccase induction was likely caused by protective responses to oxidative stress and nutrient competition during interspecific fungal interactions.