Inhibitory effect of triptolide on lymph node metastasis in patients with non-Hodgkin lymphoma by regulating SDF-1/CXCR4 axis in vitro

• Published in: Acta pharmacologica Sinica Vol. 27; no. 11; pp. 1438 - 1446
• Main Authors: Zhang, Chun, Cui, Guo-hui, Liu, Fang, Wu, Qiu-ling, Chen, Yan
• Format: Journal Article
• Language: English
• Published: United States Nature Publishing Group 01.11.2006
• Subjects: Antineoplastic Agents, Alkylating - administration & dosage; Antineoplastic Agents, Alkylating - pharmacology; Cell Line, Tumor; Cell Proliferation - drug effects; Chemokine CXCL12 - biosynthesis; Chemokine CXCL12 - genetics; Chemotaxis - drug effects; Diterpenes - administration & dosage; Diterpenes - pharmacology; Dose-Response Relationship, Drug; Epoxy Compounds - administration & dosage; Epoxy Compounds - pharmacology; Humans; Lymph Nodes - pathology; Lymphatic Metastasis; Lymphoma, B-Cell - pathology; Lymphoma, Non-Hodgkin - metabolism; Lymphoma, Non-Hodgkin - pathology; Phenanthrenes - administration & dosage; Phenanthrenes - pharmacology; Plants, Medicinal - chemistry; Receptors, CXCR4 - metabolism; RNA, Messenger - biosynthesis; RNA, Messenger - genetics; Stromal Cells - metabolism; Tripterygium - chemistry; 肿瘤转移; 雷公藤内酯; 非霍奇金淋巴瘤
• ISSN: 1671-4083; 1745-7254
• DOI: 10.1111/j.1745-7254.2006.00415.x

Aim： To investigate the antiproliferative effect of triptolide on B-NHL cell line Raji cells, to study its effect on lymph node metastasis in patients with non-Hodgkin＇s lymphoma （NHL） in vitro, and to explore the underlying mechanism regulating SDF-1/CXCR4 axis. Methods： The effects of triptolide on the growth of Raji cells were studied by 3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2H-tetrazolium （MTT） assay. The effects of triptolide on SDF-1 mRNA expression in lymph node stromal cells from patients with NHL were determined by reverse transcriptase-polymerase chain reaction （RT-PCR）. The effects of triptolide on CXCR4 expression on lym- phoma cells freshly isolated from the lymph nodes of these patients were studied by flow cytometric analysis. Chemotaxis assays were performed to observe the effects of triptolide on migration of primary lymphoma cells towards recombinant human SDF-1α （rhSDF-1α） or cultured lymph node stromal cells in vitro. Results： Triptolide inhibited the proliferation of B-NHL cell line Raji cells in a dose- and time-dependent manner with a 24-h IC50 value of 43.06 nmol/L and a 36-h IC50 value of 25.08 nmol/L. The expression of SDF-1α mRNA in lymph node stromal cells obtained from patients with NHL was decreased after treatment by triptolide at concentrations of 25 and 50 nmol/L for 24 h. Flow cytometry analysis showed that the CXCR4 expression on primary lymphoma cells were downregulated gradually in a dose-dependent manner following triptolide treatment. Chemotaxis assays revealed that the migration of freshly isolated lymphoma cells towards either rhSDF-1 or cultured lymph node stromal cells was markedly inhibited by the addition of triptolide in vitro, and the inhibition was dose-dependent. Conclusion： Triptolide can inhibit the proliferation of B-NHL cell line Raji cells. Moreover, triptolide is able to inhibit the migration of lymphoma cells via lymph nodes in vitro. The potential antitumor mechanisms of triptolide are related to the antiproliferative effect and the blockage of SDF-1/CXCR4 axis.