Proteomic characterization of medicinal plants used in the treatment of diabetes

Several plants have been studied for their medicinal properties, especially concerning the management of chronic diseases, such as diabetes, aiming at a more accessible form of treatment. In this context, the aim of this study was to characterize plant proteins used in folk medicine as hypoglycemic...

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Published inInternational journal of biological macromolecules Vol. 140; pp. 294 - 302
Main Authors Pedrete, Thaís A., Hauser-Davis, Rachel A., Moreira, Josino C.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.11.2019
Subjects
Bauhinia - metabolism
Brazil
Chromatography, Liquid
Chrysobalanaceae - metabolism
Diabetes
Diabetes Mellitus - drug therapy
Geography
Hypoglycemic Agents - chemistry
Hypoglycemic Agents - pharmacology
Medicinal plants
Medicine, Traditional
Plant Extracts - chemistry
Plant Extracts - pharmacology
Plant Leaves - metabolism
Plants, Medicinal
Proteome
Proteomics
Tandem Mass Spectrometry
Brazil
Medicinal plants
2DE
MA
MS
PG
RMA
LC
Proteomics
FASP
TOF
AL
Diabetes
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Summary:Several plants have been studied for their medicinal properties, especially concerning the management of chronic diseases, such as diabetes, aiming at a more accessible form of treatment. In this context, the aim of this study was to characterize plant proteins used in folk medicine as hypoglycemic agents for the treatment of diabetes, namely “abajerú” (Chrysobalanus icaco) and “cow's paw” (Bauhinia forficata and Bauhinia variegata). The species were differentiated by proteome characterization. Proteins were in-solution digested using trypsin by the filter-assisted sample preparation (FASP) method. Peptides were then analyzed by liquid chromatography tandem mass spectrometry (LC–MS/MS) for protein characterization. In total, 131 proteins were identified. The main biological functions of these proteins were cellular respiration, transport, metabolism and photosynthesis. Insulin-like proteins were not detected, but proteins involved in controlling glucose levels were. The results are of value in the proteomic characterization of phytotherapeutic plants, and may serve as baseline for the assessed species in Brazil, where a lack of knowledge in this regard is observed.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2019.08.035