Total internal reflection with fluorescence correlation spectroscopy: Applications to substrate-supported planar membranes

In this paper, the conceptual basis and experimental design of total internal reflection with fluorescence correlation spectroscopy (TIR-FCS) is described. The few applications to date of TIR-FCS to supported membranes are discussed, in addition to a variety of applications not directly involving su...

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Bibliographic Details
Published inJournal of structural biology Vol. 168; no. 1; pp. 95 - 106
Main Authors Thompson, Nancy L., Wang, Xiang, Navaratnarajah, Punya
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.10.2009
Subjects
Cell Membrane - chemistry
Cell membranes
Diffusion
Evanescent
Fluctuation
Fluorescence microscopy
Membranes, Artificial
Models, Theoretical
Optical microscopy
Single molecule
Spectrometry, Fluorescence - methods
Supported membranes
Surface binding
Surface Plasmon Resonance
Optical microscopy
Supported membranes
Surface binding
Evanescent
Fluctuation
Cell membranes
Diffusion
Fluorescence microscopy
Single molecule
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Summary:In this paper, the conceptual basis and experimental design of total internal reflection with fluorescence correlation spectroscopy (TIR-FCS) is described. The few applications to date of TIR-FCS to supported membranes are discussed, in addition to a variety of applications not directly involving supported membranes. Methods related, but not technically equivalent, to TIR-FCS are also summarized. Future directions for TIR-FCS are outlined.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:1047-8477
1095-8657
DOI:10.1016/j.jsb.2009.02.013