Production of a Biologically Active Epidermal Growth Factor Fusion Protein with High Collagen Affinity

• Published in: Journal of biochemistry (Tokyo) Vol. 129; no. 4; pp. 627 - 633
• Main Authors: Ishikawa, Tetsuya, Terai, Hiroshi, Kitajima, Takashi
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.04.2001
• Subjects: Animals; Binding, Competitive; Blotting, Western; Cell Division - drug effects; Cell Line; Collagen - antagonists & inhibitors; Collagen - metabolism; collagen-binding; Diabetes Mellitus - genetics; Dose-Response Relationship, Drug; epidermal growth factor; Epidermal Growth Factor - metabolism; Epidermal Growth Factor - pharmacology; fibronectin; fusion protein; Gelatin - metabolism; Immunohistochemistry; Mice; Protein Binding; Rats; Recombinant Fusion Proteins - metabolism; Recombinant Fusion Proteins - pharmacology; Substrate Specificity; Thermodynamics; wound healing; Wounds and Injuries - metabolism
• ISSN: 0021-924X; 1756-2651
• DOI: 10.1093/oxfordjournals.jbchem.a002900

Collagen is generally incapable of capturing polypeptides such as growth factors in a specific manner. In this study, we established a collagen-binding growth factor (FNCBDEGF) consisting of epidermal growth factor (EGF) and the fibronectin collagen-binding domain. A typical yield of FNCBD-EGF was approximately 200 μg/ml culture in an Escherichia coli expression system. This fusion protein bound to gelatin and fibrillar collagen sponges, and the bound protein was not effectively eluted even with 2 M NaCl. In addition, FNCBD-EGF bound to type I, II, HI, or IV collagen-coated plates, and the specificity of binding was confirmed by competitive inhibition using fibronectin. FNCBD-EGF substantially stimulated cell growth after binding to collagen-coated culture plates, whereas EGF had no effect, indicating that this fusion protein acted as a collagen- associated growth factor. In an animal model of impaired wound healing, FNCBDEGF, but not EGF, was retained with collagen sponges at wound sites 4 d after implantation, and repair of epidermis was observed underneath the sponges. These results suggested that our fusion protein with high collagen affinity would be useful for wound healing.