Effect of carbohydrate-protein supplementation postexercise on rat muscle glycogen synthesis and phosphorylation of proteins controlling glucose storage

• Published in: Metabolism, clinical and experimental Vol. 60; no. 10; pp. 1406 - 1415
• Main Authors: Hara, Daisuke, Morrison, Paul J, Ding, Zhenping, Ivy, John L
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.10.2011; Elsevier
• Subjects: Animals; Biological and medical sciences; Blood Glucose - metabolism; Dietary Carbohydrates - administration & dosage; Dietary Carbohydrates - pharmacology; Dietary Proteins - administration & dosage; Dietary Proteins - pharmacology; Dietary Supplements; Drug Administration Schedule; Endocrinology & Metabolism; Feeding. Feeding behavior; Fundamental and applied biological sciences. Psychology; Glucose - metabolism; Glycogen - biosynthesis; Glycogen - metabolism; Insulin - blood; Male; Muscle Proteins - metabolism; Muscle Proteins - physiology; Muscle, Skeletal - drug effects; Muscle, Skeletal - metabolism; Phosphorylation - drug effects; Physical Conditioning, Animal - physiology; Rats; Rats, Sprague-Dawley; Time Factors; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Phosphorylation; Rat; Glycogen; Rodentia; Glucose; Protein; Vertebrata; Mammalia; Storage; Animal; Carbohydrate; Muscle; Supplementation; Endocrinology
• ISSN: 0026-0495; 1532-8600
• DOI: 10.1016/j.metabol.2011.02.007

Abstract To examine whether addition of protein to a carbohydrate supplement enhances muscle glycogen synthesis, we compared the muscle glycogen concentrations of rats that had been depleted of their muscle glycogen stores with a 3-hour swim and immediately supplemented with a placebo (Con), carbohydrate (CHO), or carbohydrate plus protein supplement (C+P). Rats were given either 0.9 g carbohydrate per kilogram body mass for the CHO group or 0.9 g carbohydrate + 0.3 g protein per kilogram body mass for the C+P groups. Muscle samples of the red and white quadriceps were excised immediately, 30 minutes, or 90 minutes postexercise. Glycogen concentration of the C+P group was greater than that of the CHO group at 90 minutes postexercise in both red (C+P, 28.3 ± 2.6 µ mol/g vs CHO, 22.4 ± 2.0 µ mol/g; P < .05) and white (C+P, 24.9 ± 2.4 µ mol/g vs CHO, 17.64 ± 1.5 µ mol/g; P < .01) quadriceps. Protein kinase B phosphorylation was greater in the C+P-30 group (the number following treatment group abbreviation refers to time [in minutes] of euthanasia following exercise) than the sedentary control and exercised control groups in red quadriceps at 30 minutes and in white quadriceps at 90 minutes postexercise. This difference was not observed in the CHO group. Phosphorylation of glycogen synthase was significantly reduced 30 minutes postexercise and returned to baseline levels by 90 minutes postexercise in both CHO- and C+P-supplemented groups, with no difference between supplements. These results demonstrated that the addition of protein to a carbohydrate supplement will enhance the rate of muscle glycogen restoration postexercise and may involve facilitation of the glucose transport process.