Metabolic heterogeneity of nuclear poly (A)-containing RNA in mouse liver

SUMMARY The analysis by the approach to equilibrium labeling method has shown that the poly(A)+ fraction of liver hnRNA is not a uniform class of molecules, but is comprised of two distinct subclasses with half-lives of 5 and 60 min, while the poly(A)− hnRNA was metabolically homogeneous and turned...

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Bibliographic Details
Published inNucleic acids research Vol. 4; no. 10; pp. 3473 - 3482
Main Authors Chemovskaya, T. V., Lerman, M. I.
Format Journal Article
LanguageEnglish
Published England Oxford University Press 01.10.1977
Subjects
Animals
Half-Life
Kinetics
Liver - metabolism
Male
Mice
Orotic Acid - metabolism
Poly A - metabolism
RNA - metabolism
RNA, Messenger - metabolism
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Summary:SUMMARY The analysis by the approach to equilibrium labeling method has shown that the poly(A)+ fraction of liver hnRNA is not a uniform class of molecules, but is comprised of two distinct subclasses with half-lives of 5 and 60 min, while the poly(A)− hnRNA was metabolically homogeneous and turned over with a rather uniform half-life of 30 min. The results suggest that (a) poly(A) synthesis and addition is not limiting for the rate of hnRNA processing, and (b) there is a correlation between the kinetics of mRNA appearance in the cytoplasm and kinetic behaviour of their possible nuclear precursors.
Bibliography:istex:43EB7B63044170C601A0EAA0B202111A9AD862FF
ArticleID:4.10.3473
ark:/67375/HXZ-T15PBKD2-D
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/4.10.3473