Influence of weighted downhill running training on serial sarcomere number and work loop performance in the rat soleus

• Published in: Biology open Vol. 11; no. 7
• Main Authors: Hinks, Avery, Jacob, Kaitlyn, Mashouri, Parastoo, Medak, Kyle D., Franchi, Martino V., Wright, David C., Brown, Stephen H. M., Power, Geoffrey A.
• Format: Journal Article
• Language: English
• Published: London The Company of Biologists Ltd 01.07.2022; The Company of Biologists
• Subjects: Body mass; Collagen; Crosslinking; dynamic performance; eccentric training; force-length relationship; Hydroxyproline; Hypotheses; muscle architecture; Muscle contraction; Muscles; Musculoskeletal system; Pepsin; Physiology; Running; sarcomerogenesis; Training
• ISSN: 2046-6390; 2046-6390
• DOI: 10.1242/bio.059491

ABSTRACT Increased serial sarcomere number (SSN) has been observed in rats following downhill running training due to the emphasis on active lengthening contractions; however, little is known about the influence on dynamic contractile function. Therefore, we employed 4 weeks of weighted downhill running training in rats, then assessed soleus SSN and work loop performance. We hypothesised trained rats would produce greater net work output during work loops due to a greater SSN. Thirty-one Sprague-Dawley rats were assigned to a training or sedentary control group. Weight was added during downhill running via a custom-made vest, progressing from 5–15% body mass. Following sacrifice, the soleus was dissected, and a force-length relationship was constructed. Work loops (cyclic muscle length changes) were then performed about optimal muscle length (LO) at 1.5–3-Hz cycle frequencies and 1–7-mm length changes. Muscles were then fixed in formalin at LO. Fascicle lengths and sarcomere lengths were measured to calculate SSN. Intramuscular collagen content and crosslinking were quantified via a hydroxyproline content and pepsin-solubility assay. Trained rats had longer fascicle lengths (+13%), greater SSN (+8%), and a less steep passive force-length curve than controls (P<0.05). There were no differences in collagen parameters (P>0.05). Net work output was greater (+78–209%) in trained than control rats for the 1.5-Hz work loops at 1 and 3-mm length changes (P<0.05), however, net work output was more related to maximum specific force (R2=0.17-0.48, P<0.05) than SSN (R2=0.03-0.07, P=0.17-0.86). Therefore, contrary to our hypothesis, training-induced sarcomerogenesis likely contributed little to the improvements in work loop performance. This article has an associated First Person interview with the first author of the paper.