Analysis of short-chain fatty acids in human feces: A scoping review

Short-chain fatty acids (SCFAs) play a crucial role in maintaining homeostasis in humans, therefore the importance of a good and reliable SCFAs analytical detection has raised a lot in the past few years. The aim of this scoping review is to show the trends in the development of different methods of...

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Bibliographic Details
Published inAnalytical biochemistry Vol. 526; pp. 9 - 21
Main Authors Primec, Maša, Mičetić-Turk, Dušanka, Langerholc, Tomaž
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.06.2017
Subjects
capillary electrophoresis
Chromatography, Gas - methods
Chromatography, High Pressure Liquid - methods
cost effectiveness
Detection methods
Fatty Acids, Volatile - analysis
Feces
Feces - chemistry
gas chromatography
high performance liquid chromatography
homeostasis
Human
Humans
in vivo studies
nuclear magnetic resonance spectroscopy
Scoping review
short chain fatty acids
Short-chain fatty acids (SCFAs)
SPE
IBD
PDA
FFAP
Short-chain fatty acids (SCFAs)
SPME
FAs
ESI
Feces
IBS
GC
Detection methods
HPLC
Human
FID
LOD
UV
CE
RSD
VWD
ECD
LOQ
RP-HPLC
NMR
FAME
PEG
CV
LC
RI
Scoping review
SCFAs
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Summary:Short-chain fatty acids (SCFAs) play a crucial role in maintaining homeostasis in humans, therefore the importance of a good and reliable SCFAs analytical detection has raised a lot in the past few years. The aim of this scoping review is to show the trends in the development of different methods of SCFAs analysis in feces, based on the literature published in the last eleven years in all major indexing databases. The search criteria included analytical quantification techniques of SCFAs in different human clinical and in vivo studies. SCFAs analysis is still predominantly performed using gas chromatography (GC), followed by high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) and capillary electrophoresis (CE). Performances, drawbacks and advantages of these methods are discussed, especially in the light of choosing a proper pretreatment, as feces is a complex biological material. Further optimization to develop a simple, cost effective and robust method for routine use is needed.
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ISSN:0003-2697
1096-0309
1096-0309
DOI:10.1016/j.ab.2017.03.007