Chitosan-Based Bioactive Glass Gauze: Microstructural Properties, In Vitro Bioactivity, and Biological Tests

Passive commercial gauzes were turned into interactive wound dressings by impregnating them with a chitosan suspension. To further improve healing, and cell adhesion and proliferation, chitosan/bioactive glass wound dressings were produced with the addition of (i) 45S5, (ii) a Sr- and Mg-containing...

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Published inMaterials Vol. 13; no. 12; p. 2819
Main Authors Sergi, Rachele, Bellucci, Devis, Salvatori, Roberta, Cannillo, Valeria
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 23.06.2020
MDPI
Subjects
Bacteria
Bioglass
Biological activity
biological tests
Biomedical materials
Body fluids
Cell adhesion
Cell adhesion & migration
Cell growth
Chitosan
chitosan/bioactive glass wound dressings
Collagen
cytotoxicity
Extracellular matrix
Fibroblasts
Gauze
Impregnation
In vitro methods and tests
Magnesium
Morphology
SBF
scratch test
Scratch tests
Skin
Spectrum analysis
Wound healing
Zinc
Italy
Germany
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Summary:Passive commercial gauzes were turned into interactive wound dressings by impregnating them with a chitosan suspension. To further improve healing, and cell adhesion and proliferation, chitosan/bioactive glass wound dressings were produced with the addition of (i) 45S5, (ii) a Sr- and Mg-containing bioactive glass, and (iii) a Zn-containing bioactive glass to the chitosan suspension. SEM and FTIR analyses evidenced positive results in terms of incorporation of bioactive glass particles. Bioactivity was investigated by soaking chitosan-based bioactive glass wound dressings in simulated body fluid (SBF). Cell viability, proliferation, and morphology were investigated using NIH 3T3 (mouse embryonic fibroblast) cells by neutral red (NR) uptake and MTT assays. Furthermore, the wound-healing rate was evaluated by means of the scratch test, using NIH 3T3. The results showed that bioactive glass particles enhance cell adhesion and proliferation, and wound healing compared to pure chitosan. Therefore, chitosan-based bioactive glass wound dressings combine the properties of the organic matrix with the specific biological characteristics of bioactive glasses to achieve chitosan composites suitable for healing devices.
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ISSN:1996-1944
1996-1944
DOI:10.3390/ma13122819