In vitro evaluation of ivermectin, moxidectin, albendazole and pyrantel against cyathostomins of horses

• Published in: Revista brasileira de parasitologia veterinaria Vol. 27; no. 1; pp. 91 - 93
• Main Authors: Molento, Marcelo Beltrão, Canever, Ricardo José
• Format: Journal Article
• Language: English
• Published: Brazil Colégio Brasileiro de Parasitologia Veterinária 01.01.2018; Colégio Brasileiro de Parasitologia Veterinaria
• Subjects: Albendazole - pharmacology; Animals; anti-helmínticos; Antiparasitic Agents - pharmacology; Cavalos; Horses - parasitology; Ivermectin - pharmacology; Larva - drug effects; Macrolides - pharmacology; Nematoda - drug effects; PARASITOLOGY; Parasitology - methods; parasitos; Pyrantel - pharmacology; teste in vitro; VETERINARY SCIENCES; Cavalos; Horse; parasitos; Anthelmintics; parasites; In vitro test; anti-helmínticos; teste in vitro
• ISSN: 0103-846X; 1984-2961; 1984-2961
• DOI: 10.1590/S1984-29612017055

Cyathostomins are the most prevalent nematodes of horses, and multidrug resistance has been reported worldwide. There is a need to implement alternative drug monitoring analytical tests. The objective of this study was to determine the consistency (5 repetitions) of the larval migration on agar test (LMAT) using ivermectin, moxidectin, pyrantel or albendazole against cyathostomin infective-stage larvae in eight different concentrations. LMAT showed a strong coefficient of determination (R2 > 0.91), between the test repetitions (n=5). The average 50% effective concentration (EC50) for ivermectin, moxidectin, pyrantel and albendazole were 0.0404, 0.0558, 0.0864 and 0.0988 nMol, respectively. The results of the EC50 for albendazole showed the greatest range of concentration. Ivermectin and moxidectin had the lowest in between-test variation. In the future, internationally certified susceptible isolates could be used for screening new drug candidates, or to follow up the pattern of drug efficacy from field populations.