Gene expression in chorionic villous samples at 11 weeks' gestation from women destined to develop preeclampsia

• Published in: Prenatal diagnosis Vol. 28; no. 10; pp. 956 - 961
• Main Authors: Farina, Antonio, Sekizawa, Akihiko, De Sanctis, Paola, Purwosunu, Yuditiya, Okai, Takashi, Cha, Dong Hyun, Kang, Jin Hee, Vicenzi, Claudia, Tempesta, Annalisa, Wibowo, Noroyono, Valvassori, Luisella, Rizzo, Nicola
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.10.2008; Wiley
• Subjects: Adult; Antigens, CD - genetics; Biological and medical sciences; Biomarkers; Case-Control Studies; Chorionic Villi Sampling; chorionic villous samples; Delivery. Postpartum. Lactation; Endoglin; Female; Fundamental and applied biological sciences. Psychology; Gene Expression; Genetics of eukaryotes. Biological and molecular evolution; Gynecology. Andrology. Obstetrics; Heme Oxygenase-1 - genetics; Humans; Medical sciences; Molecular and cellular biology; mRNA; Pre-Eclampsia - diagnosis; Pre-Eclampsia - genetics; preeclampsia; Pregnancy; Pregnancy Trimester, First - genetics; Prospective Studies; real-time PCR; Receptors, Cell Surface - genetics; RNA, Messenger - genetics; Statistics, Nonparametric; Superoxide Dismutase - genetics; Transforming Growth Factor beta1 - genetics; Human; Pregnancy disorders; Sample; Gynecology; Neonatology; chorionic villous samples; mRNA; Chorion; real-time PCR; Gene expression; Real time; Pregnancy toxemia; Pregnancy; Polymerase chain reaction; Messenger RNA; Preeclampsia; Female; Genetics; Molecular biology; Woman
• ISSN: 0197-3851; 1097-0223
• DOI: 10.1002/pd.2109

Objective To evaluate the direct alterations in mRNA expression among chorionic villous samples from 11 weeks' pregnant women who would develop preeclampsia (PE) later in the pregnancy. Method Case‐control study encompassing five women destined to develop PE [cases matched 1:5 for gestational age (GA) with 25 controls]. We quantified mRNA expression on tissue samples from chorionic villous sampling (CVS) of normal and PE patients. We then assessed mRNA expressions of vascular endothelial growth factor (VEGFA), VEGFA receptor 1 (Flt‐1), endoglin (Eng), placental growth factor (PlGF), transforming growth factor‐β1 (TGF‐β1), heme oxygenase‐1 (HO‐1) and superoxide dismutase (SOD). Data were analyzed by nonparametric rank analysis. Results For all the mRNA species considered in this study, all the mean observed ranks in the PE group were significantly altered compared to the rank expectation among controls. mRNA for Eng and TGF‐β1 were the markers with the highest degree of aberration in PE, in respect to controls. The results are consistent with those already reported for the corresponding circulating proteins. mRNA for HO‐1 and SOD were instead associated with the lowest aberration. Conclusion It is assumed that the pathogenesis of PE is associated with pathophysiological alterations to trophoblasts in early gestation. Our study has directly proved that gene expressions relating to angiogenesis or oxidative stress are altered in the first trimester trophoblasts that go on to develop PE later. These results would put the basis for a possible screening method for PE by using residual CVS. Copyright © 2008 John Wiley & Sons, Ltd.