Phenotypically and functionally distinct subsets of natural killer cells in human PBMCs
Human natural killer (NK) cells are one major component of lymphocytes that mediate early protection against viruses and tumor cells, and play an important role in immune regulatory functions. In this study, we demonstrated that human NK cells could be divided into four subsets, CD56 hiCD16 −, CD56...
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Published in | Cell biology international Vol. 32; no. 2; pp. 188 - 197 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Elsevier Ltd
01.02.2008
Blackwell Publishing Ltd |
Subjects | |
Online Access | Get full text |
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Summary: | Human natural killer (NK) cells are one major component of lymphocytes that mediate early protection against viruses and tumor cells, and play an important role in immune regulatory functions. In this study, we demonstrated that human NK cells could be divided into four subsets, CD56
hiCD16
−, CD56
loCD16
−, CD56
+CD16
+ and CD56
−CD16
+, based on the expression of cell surface CD56 and CD16 molecules. Phenotypic analysis of NK cell subsets indicated that the expression of activation markers, adhesion molecules, memory cell markers, inhibitory and activating receptors, and intracellular proteins (granzyme B and perforin) were heterogeneous. Following interleukin (IL)-2 stimulation, interferon-γ was preferentially produced by CD56
+CD16
− NK cells and this subset showed more proliferative capacity. The cytolytic activity of both CD56
+CD16
− and CD56
+/−CD16
+ subsets could be augmented in response to IL-2. The data provided a new definition for NK cell subsets demonstrating their phenotypic and functional diversity and possible stage of NK cell differentiation in peripheral blood. |
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Bibliography: | ArticleID:CBIN2589 ark:/67375/WNG-MDWP3P7J-N istex:576DA8C5989892D3F7B663B1F96238841442BCDE ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1065-6995 1095-8355 |
DOI: | 10.1016/j.cellbi.2007.08.025 |