Electrochemical Detection of Single A-G Mismatch Using Biosensing Surface Based on Gold Nanoparticles

• Published in: Genomics, proteomics & bioinformatics Vol. 3; no. 1; pp. 47 - 51
• Main Authors: Zhang, Ren-Yun, Wang, Xue-Mei, Gong, Sheng-Jin, He, Nong-Yue
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 2005; Chien-Shiung Wu Laboratory, National Lab of Molecular and Biomolecular Electronics, Department of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China; Elsevier
• Subjects: A-G基因; Base Pair Mismatch; Biosensing Techniques; biosensor; Dacarbazine - metabolism; DTIC; Electrochemistry; Gold; gold nanoparticles; Indicators and Reagents; Letter; Nanostructures; Oligonucleotides - metabolism; single base mismatch; 基因错配; 抗癌药物; 电气化学; 纳米微粒; 药物反应; DTIC; single base mismatch; biosensor; electrochemistry; gold nanoparticles
• ISSN: 1672-0229; 2210-3244
• DOI: 10.1016/S1672-0229(05)03007-X

The study of small drug molecules interacting with nucleic acids is an area of intense research that has particular relevance in our understanding of relative mechanism in chemotherapeutic applications and the association between genetics （including sequence variation） and drug response. In this contribution, we demonstrate how the sequence-specific binding of an anticancer drug Dacarbazine （DTIC） to single base （A-G） mismatch could be sensitively detected by combining electrochemical detection with biosensing surface based on gold nanoparticles.