Flowering time regulator qFT13‐3 involved in soybean adaptation to high latitudes

• Published in: Plant biotechnology journal Vol. 22; no. 5; pp. 1164 - 1176
• Main Authors: Li, Yan‐fei, Zhang, Liya, Wang, Jun, Wang, Xing, Guo, Shiyu, Xu, Ze‐jun, Li, Delin, Liu, Zhangxiong, Li, Ying‐hui, Liu, Bin, Qiu, Li‐juan
• Format: Journal Article
• Language: English
• Published: England John Wiley and Sons Inc 01.05.2024
• Subjects: adaptation; artificial selection; biotechnology; circadian clocks; cultivars; data collection; domestication; flowering time; Flowers - genetics; Gene Expression Regulation, Plant - genetics; genes; Genome-Wide Association Study; Glycine max - genetics; haplotypes; Haplotypes - genetics; landraces; latitude; Photoperiod; Plant Breeding; Plant Proteins - genetics; soybean; soybeans; transcription factors; yield‐related traits; soybean; adaptation; genome‐wide association study; flowering time; yield‐related traits
• ISSN: 1467-7644; 1467-7652; 1467-7652
• DOI: 10.1111/pbi.14254

Summary Soybean is a short‐day plant that typically flowers earlier when exposed to short‐day conditions. However, the identification of genes associated with earlier flowering time but without a yield penalty is rare. In this study, we conducted genome‐wide association studies (GWAS) using two re‐sequencing datasets that included 113 wild soybeans (G. soja) and 1192 cultivated soybeans (G. max), respectively, and simultaneously identified a candidate flowering gene, qFT13‐3, which encodes a protein homologous to the pseudo‐response regulator (PRR) transcription factor. We identified four major haplotypes of qFT13‐3 in the natural population, with haplotype H4 (qFT13‐3H4) being lost during domestication, while qFT13‐3H1 underwent natural and artificial selection, increasing in proportion from 4.5% in G. soja to 43.8% in landrace and to 81.9% in improve cultivars. Notably, most cultivars harbouring qFT13‐3H1 were located in high‐latitude regions. Knockout of qFT13‐3 accelerated flowering and maturity time under long‐day conditions, indicating that qFT13‐3 functions as a flowering inhibitor. Our results also showed that qFT13‐3 directly downregulates the expression of GmELF3b‐2 which is a component of the circadian clock evening complex. Field trials revealed that the qft13‐3 mutants shorten the maturity period by 11 days without a concomitant penalty on yield. Collectively, qFT13‐3 can be utilized for the breeding of high‐yield cultivars with a short maturity time suitable for high latitudes.