On the search for glycated lipoprotein ApoA-I in the plasma of diabetic and nephropathic patients

• Published in: Journal of mass spectrometry. Vol. 43; no. 1; pp. 74 - 81
• Main Authors: Lapolla, Annunziata, Brioschi, Maura, Banfi, Cristina, Tremoli, Elena, Bonfante, Luciana, Cristoni, Simone, Seraglia, Roberta, Traldi, Pietro
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.01.2008; Wiley
• Subjects: 2D gel electrophoresis; Amino Acid Sequence; Apolipoprotein A-I - blood; Apolipoprotein A-I - chemistry; Biological and medical sciences; blood serum; cholesterol; Creatinine - blood; desorption; diabetes; Diabetes Mellitus, Type 2 - blood; Electrophoresis, Gel, Two-Dimensional; glycation; Glycation End Products, Advanced - chemistry; Glycosylation; Humans; Investigative techniques, diagnostic techniques (general aspects); ionization; Kidney Failure, Chronic - blood; lipoproteins; MALDI/MS; mass spectrometry; Medical sciences; Middle Aged; Miscellaneous. Technology; Molecular Sequence Data; nephropathy; nonenzymatic glycation; obesity; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; patients; retinol-binding protein; risk; sampling; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; two-dimensional gel electrophoresis; Endocrinopathy; Biological fluid; Chemical analysis; Peptides; Enzymatic hydrolysis; Apolipoprotein AI; Matrix assisted laser desorption ionization; Glycation; Blood; Blood plasma; Clinical biology; Qualitative analysis; Aminoacid sequence; Kidney disease; Human; Glycated protein; Urinary system disease; Gel electrophoresis; Diabetes mellitus; Post source decay technique; Maillard reaction; Patient; Primary structure; Normal; nonenzymatic glycation; 2D gel electrophoresis; Peptide map; Analysis method; Nephropathy; Peptide fragment; Time of flight method; MALDI/MS; Two dimensional electrophoresis; Mass spectrometry; diabetes; Comparative study; Structural analysis
• ISSN: 1076-5174; 1096-9888
• DOI: 10.1002/jms.1274

The analysis of plasma samples from healthy, diabetic and nephropathic subjects was carried out by 2D gel electrophoresis. This approach shows clear differences among the three classes of subjects. In the case of diabetic and nephropathic patients intense spots appear. Their enzymatic digestion followed by matrix assisted laser desorption ionization/mass spectrometry (MALDI/MS) analysis shows that an overexpression of unglycated and glycated ApoA‐I is present in both pathological states. Interestingly, this trend is also observed for the retinol‐binding protein (RBP). The data obtained can be relevant to assess possible risks associated either with the glycation level of ApoA‐I or with the overexpression of RBP. In fact, in the former case possibly a different functionality of the glycated protein is to be expected, reflecting a different efficiency in cholesterol transport. In the latter case, the increase of RBP level can be related to the overweight of the diabetic subjects under investigation: it is known that obesity leads to RBP overexpression. In the case of nephropathic patients, the RBP level increases in parallel with serum creatinin. Copyright © 2007 John Wiley & Sons, Ltd.