Real‐time in vivo imaging of adult Zebrafish brain using optical coherence tomography

We report noninvasive imaging of the brain of adult Zebrafish (Danio rerio) using real time optical coherence tomography (OCT) capable of acquiring cross sectional 2D OCT images @ 8 frames/sec. Anatomic features such as telencephalon, tectum opticum, eminentia Granularis and cerebellum were clearly...

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Bibliographic Details
Published inJournal of biophotonics Vol. 2; no. 5; pp. 288 - 291
Main Authors Rao, K. Divakar, Alex, Aneesh, Verma, Yogesh, Thampi, Sreeja, Gupta, Pradeep K.
Format Journal Article
LanguageEnglish
Published Berlin WILEY‐VCH Verlag 01.05.2009
Subjects
3‐D imaging
Animals
Brain - anatomy & histology
Brain - cytology
Imaging, Three-Dimensional
Optical coherence tomography
Time Factors
Tomography, Optical Coherence - methods
Zebrafish
Zebrafish - anatomy & histology
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Summary:We report noninvasive imaging of the brain of adult Zebrafish (Danio rerio) using real time optical coherence tomography (OCT) capable of acquiring cross sectional 2D OCT images @ 8 frames/sec. Anatomic features such as telencephalon, tectum opticum, eminentia Granularis and cerebellum were clearly resolved in the OCT images. A 3D model of Zebrafish brain was reconstructed, for the first time to our knowledge, using these 2D OCT images. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
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ISSN:1864-063X
1864-0648
DOI:10.1002/jbio.200910032