Regulation of Human hst Expression by an Enhancer Element Residing in the Third Exon

• Published in: Cancer science Vol. 82; no. 11; pp. 1191 - 1195
• Main Authors: Sasaki, Akio, Kubo, Mitsumasa, Hasan, Shahid, Yano, Yoke, Mitsuaki, Mitsuaki
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.11.1991; Japanese Cancer Association; John Wiley & Sons, Inc
• Subjects: 3T3 Cells; Acetyltransferase; Animals; Biological and medical sciences; CAT assay; Chloramphenicol; Chloramphenicol O-acetyltransferase; Chloramphenicol O-Acetyltransferase - genetics; Chloramphenicol O-Acetyltransferase - metabolism; Deoxyribonuclease; Deoxyribonuclease I; Developmental stages; DNase I hypersensitivity; Enhancer Elements, Genetic; Exons; Fibroblast Growth Factor 4; Fibroblast Growth Factors - genetics; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Regulation; Growth Substances - genetics; hst; Humans; Mice; Molecular and cellular biology; Molecular genetics; mRNA; Oncogene Proteins - genetics; Oncogenes; Peptide Chain Initiation, Translational; Promoter Regions, Genetic; Proto-Oncogene Proteins - genetics; Restriction Mapping; RNA, Messenger - genetics; RNA, Messenger - metabolism; Transcription, Genetic; Translation initiation; Tumor cell lines; Human; Regulation(control); Enhancer sequence; Exon; Cell line; Tumor; Gene organization; Regulatory sequence; Gene expression; Embryonal carcinoma
• ISSN: 0910-5050; 1347-9032; 1349-7006; 1876-4673
• DOI: 10.1111/j.1349-7006.1991.tb01778.x

The hst gene is exclusively expressed in undifferentiated embryonal carcinoma cell lines and at a limited stage of embryonal development. Two DNase I‐hypersensitive sites were mapped in the 3’region (approximately 3.5 and 4,5 kb downstream of the translations) initiation site) of the human hst gene, irrespective of the presence or absence of hst mRNA in the cells. A DNA fragment containing one of these DNase I‐hypersensitive sites (at around 3.5 kb relative to the translational initiation site) showed enhancer activity when tested by chloramphenicol acetyltransferase (CAT) assay. These results strongly suggest that an enhancer element(s) exists in the third exon of the hst and that the expression of the hst may be regulated by the presence or absence of a putative protein factor(s) which binds to the enhancer.