Closure effects on oxygen transfer and aerobic growth in shake flasks

• Published in: Biotechnology and bioengineering Vol. 95; no. 1; pp. 15 - 21
• Main Authors: Nikakhtari, Hossein, Hill, Gordon A.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 05.09.2006; Wiley; Wiley Subscription Services, Inc
• Subjects: Aerobiosis - physiology; Biochemistry; Biological and medical sciences; Biological Transport, Active - physiology; Bioreactors - microbiology; Biotechnology; Cell Culture Techniques - methods; Cell Proliferation; closures; Computer Simulation; Fundamental and applied biological sciences. Psychology; Metabolism; microbial growth; Microorganisms; modeling; Models, Biological; Motion; Oxygen; Oxygen - metabolism; Oxygen Consumption - physiology; oxygen transfer; Pseudomonas putida; Pseudomonas putida - cytology; Pseudomonas putida - physiology; shake flask; Microorganism growth; closures; Growth; shake flask; Oxygen transfer; Aerobe; Modeling; microbial growth; Flask
• ISSN: 0006-3592; 1097-0290
• DOI: 10.1002/bit.20930

Oxygen mass transfer in shake flasks is an important aspect limiting the culture of aerobic microorganisms. In this work, mass transfer of oxygen through a closure and headspace of shake flasks is investigated. New equations for prediction of kGa in shake flasks with closures are introduced. Using Pseudomonas putida, microbial growth on glucose (fast metabolism) and phenol (slow metabolism) in shake flasks with closures were studied, considering both substrate and oxygen restrictions. A combined model for oxygen mass transfer and microbial growth is shown to accurately predict experimental oxygen concentrations and oxygen yield factors during growth experiments more accurately than previous models. © 2006 Wiley Periodicals, Inc.